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Science 7 September 2001:
Vol. 293. no. 5536, pp. 1840 - 1842
DOI: 10.1126/science.1062882
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Reports

Molecular Basis for High Virulence of Hong Kong H5N1 Influenza A Viruses

Masato Hatta,1 Peng Gao,1 Peter Halfmann,1 Yoshihiro Kawaoka12*

In 1997, an H5N1 influenza A virus was transmitted from birds to humans in Hong Kong, killing 6 of the 18 people infected. When mice were infected with the human isolates, two virulence groups became apparent. Using reverse genetics, we showed that a mutation at position 627 in the PB2 protein influenced the outcome of infection in mice. Moreover, high cleavability of the hemagglutinin glycoprotein was an essential requirement for lethal infection.

1 Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA.
2 Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
*   To whom correspondence should be addressed. E-mail: kawaokay{at}svm.vetmed.wisc.edu

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