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Science 22 June 2001:
Vol. 292. no. 5525, pp. 2326 - 2329
DOI: 10.1126/science.1058289
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Reports
A Protein Antibiotic in the Phage Q Virion: Diversity in Lysis Targets
Thomas G. Bernhardt,1*
Ing-Nang Wang,1*
Douglas K. Struck,2
Ryland Young1
A2, a capsid protein of RNA phage Q , is also
responsible for host lysis. A2 blocked synthesis of murein
precursors in vivo by inhibiting MurA, the catalyst of the committed
step of murein biosynthesis. An A2-resistance mutation
mapped to an exposed surface near the substrate-binding cleft of MurA.
Moreover, purified Q virions inhibited wild-type MurA, but not the
mutant MurA, in vitro. Thus, the two small phages characterized for
their lysis strategy, Q and the small DNA phage  X174, effect host
lysis by targeting different enzymes in the multistep, universally
conserved pathway of cell wall biosynthesis.
1 Department of Biochemistry and Biophysics,
Texas A&M University, 2128 TAMU, College Station, TX 77843-2128, USA.
2 Department of Medical Biochemistry and Genetics,
Texas A&M University Health Science Center, 1114 TAMU, College Station,
TX 77843-1114, USA.
*
These authors contributed equally to this work.
To whom correspondence should be addressed. E-mail:
ryland{at}tamu.edu
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