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Science 25 May 2001:
Vol. 292. no. 5521, pp. 1546 - 1549
DOI: 10.1126/science.292.5521.1546
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Reports

Requirement of DNase II for Definitive Erythropoiesis in the Mouse Fetal Liver

Kohki Kawane,1* Hidehiro Fukuyama,1* Gen Kondoh,2 Junji Takeda,2 Yoshiyuki Ohsawa,3 Yasuo Uchiyama,3 Shigekazu Nagata1dagger

Mature erythrocytes in mammals have no nuclei, although they differentiate from nucleated precursor cells. The mechanism by which enucleation occurs is not well understood. Here we show that deoxyribonuclease II (DNase II) is indispensable for definitive erythropoiesis in mouse fetal liver. No live DNase II-null mice were born, owing to severe anemia. When mutant fetal liver cells were transferred into lethally irradiated wild-type mice, mature red blood cells were generated from the mutant cells, suggesting that DNase II functions in a non-cell-autonomous manner. Histochemical analyses indicated that the critical cellular sources of DNase II are macrophages present at the site of definitive erythropoiesis in the fetal liver. Thus, DNase II in macrophages appears to be responsible for destroying the nuclear DNA expelled from erythroid precursor cells.

1 Department of Genetics, Osaka University Medical School, and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Suita, Osaka 565-0871, Japan.
2 Department of Social and Environmental Medicine, Osaka University Medical School, Suita, Osaka 565-0871, Japan.
3 Department of Cell Biology and Anatomy, Osaka University Medical School, Suita, Osaka 565-0871, Japan.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: nagata{at}genetic.med.osaka-u.ac.jp

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