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Originally published in Science Express on 5 April 2001
Science 13 April 2001:
Vol. 292. no. 5515, pp. 288 - 290
DOI: 10.1126/science.1059497
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Reports

Regulation of Longevity and Stress Resistance by Sch9 in Yeast

Paola Fabrizio,1 Fabiola Pozza,1 Scott D. Pletcher,2 Christi M. Gendron,2 Valter D. Longo1*

The protein kinase Akt/protein kinase B (PKB) is implicated in insulin signaling in mammals and functions in a pathway that regulates longevity and stress resistance in Caenorhabditis elegans. We screened for long-lived mutants in nondividing yeast Saccharomyces cerevisiae and identified mutations in adenylate cyclase and SCH9, which is homologous to Akt/PKB, that increase resistance to oxidants and extend life-span by up to threefold. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 were required for this life-span extension. These results indicate that longevity is associated with increased investment in maintenance and show that highly conserved genes play similar roles in life-span regulation in S. cerevisiae and higher eukaryotes.

1 Division of Biogerontology, Andrus Gerontology Center, and Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0191, USA.
2 Max Planck Institute for Demographic Research, Rostock, 18057 Germany.
*   To whom correspondence should be addressed. E-mail: vlongo{at}usc.edu

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