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Originally published in Science Express on 22 February 2001
Science 23 March 2001:
Vol. 291. no. 5512, pp. 2411 - 2413
DOI: 10.1126/science.1058366
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Reports

Length of the Flagellar Hook and the Capacity of the Type III Export Apparatus

Shigeru Makishima,1 Kaoru Komoriya,2 Shigeru Yamaguchi,3 Shin-Ichi Aizawa1*

Length determination in biology generally uses molecular rulers. The hook, a part of the flagellum of motile bacteria, has an invariant length. Here, we examined hook length and found that it was determined not by molecular rulers but probably by the amount of subunit protein secreted by the flagellar export apparatus. The export apparatus shares common features with the type III virulence-factor secretion machinery and thus may be used more widely in length determination of structures other than flagella.

1 Department of Biosciences, Teikyo University, 1-1 Toyosatodai, Utsunomiya 320-8551, Japan.
2 Department of Applied Biochemistry, Utsunomiya University, Utsunomiya 321-8505, Japan.
3 Izumi Campus, Meiji University, 1-9-1 Eifuku, Suginami, Tokyo 168-0064, Japan.
*   To whom correspondence should be addressed. E-mail: aizawa{at}nasu.bio.teikyo-u.ac.jp

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