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Glycoprotein Structure Determination by Mass Spectrometry
Anne Dell,*Howard R. Morris
The human genome encodes 30,000 to 40,000 proteins, and a major
challenge is to understand how posttranslational events, suchas
glycosylation, affect the activities and functions of theseproteins in
health and disease. Glycosylated proteins are ubiquitouscomponents of
extracellular matrices and cellular surfaces wheretheir
oligosaccharide moieties are implicated in a wide rangeof cell-cell
and cell-matrix recognition events. The power ofultrahigh-sensitivity
mass spectrometric strategies for definingthe primary structures of
highly complex mixtures of glycoproteinglycoforms is set to
revolutionize structural glycobiology inthe coming postgenomic era.
Department of Biochemistry, Imperial College of Science,
Technology and Medicine, London SW7 2AY, UK.
*
To whom correspondence should be addressed. E-mail:
a.dell{at}ic.ac.uk
The editors suggest the following Related Resources on Science sites:
In Science Magazine
INTRODUCTION TO SPECIAL ISSUE
Stella Hurtley, Robert Service, and Phil Szuromi (23 March 2001) Science291 (5512), 2337.
[DOI: 10.1126/science.291.5512.2337] |Summary »
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VIEWPOINT
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