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Originally published in Science Express on 15 February 2001
Science 16 March 2001:
Vol. 291. no. 5511, pp. 2159 - 2162
DOI: 10.1126/science.1058955
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Reports

An RNA Ligase Essential for RNA Editing and Survival of the Bloodstream Form of Trypanosoma brucei

Achim Schnaufer,12 Aswini K. Panigrahi,12 Brian Panicucci,1 Robert P. Igo Jr.,12 Reza Salavati,12 Kenneth Stuart12*

RNA editing in trypanosomes occurs by a series of enzymatic steps that are catalyzed by a macromolecular complex. The TbMP52 protein is shown to be a component of this complex, to have RNA ligase activity, and to be one of two adenylatable proteins in the complex. Regulated repression of TbMP52 blocks editing, which shows that it is a functional component of the editing complex. This repression is lethal in bloodforms of the parasite, indicating that editing is essential in the mammalian stage of the life cycle. The editing complex, which is present in all kinetoplastid parasites, may thus be a chemotherapeutic target.

1 Seattle Biomedical Research Institute, Seattle, WA 98109, USA.
2 Department of Pathobiology, University of Washington, Seattle, WA 98195, USA.
*   To whom correspondence should be addressed at the Seattle Biomedical Research Institute, 4 Nickerson Street, Suite 200, Seattle, WA 98109, USA. E-mail: kstuart{at}u.washington.edu

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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »


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