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Science 23 February 2001:
Vol. 291. no. 5508, pp. 1550 - 1553
DOI: 10.1126/science.1057330
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Reports

Centrosome-Dependent Exit of Cytokinesis in Animal Cells

Matthieu Piel,1 Joshua Nordberg,2 Ursula Euteneuer,3 Michel Bornens1*

As an organelle coupling nuclear and cytoplasmic divisions, the centrosome is essential to mitotic fidelity, and its inheritance could be critical to understanding cell transformation. Investigating the behavior of the centrosome in living mitotic cells, we documented a transient and remarkable postanaphase repositioning of this organelle, which apparently controls the release of central microtubules from the midbody and the completion of cell division. We also observed that the absence of the centrosome leads to defects in cytokinesis. Together with recent results in yeasts, our data point to a conserved centrosome-dependent pathway that integrates spatial controls into the decision of completing cell division, which requires the repositioning of the centrosome organelle.

1 Institut Curie, Section Recherche, UMR 144 du CNRS, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
2 Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
3 Zellbiologie, Adolf-Butenandt-Institut, Ludwig-Maximilians-Universität, D-80336 München, Germany.
*   To whom correspondence should be addressed.

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J. Cell Biol. 161, 535-545
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Centrosomes Split in the Presence of Impaired DNA Integrity during Mitosis.
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Mol. Biol. Cell 14, 1993-2004
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Centrosome Abnormalities and Chromosome Instability Occur Together in Pre-invasive Carcinomas.
G. A. Pihan, J. Wallace, Y. Zhou, and S. J. Doxsey (2003)
Cancer Res. 63, 1398-1404
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E1A Deregulates the Centrosome Cycle in a Ran GTPase-dependent Manner.
A. De Luca, R. Mangiacasale, A. Severino, L. Malquori, A. Baldi, A. Palena, A. M. Mileo, P. Lavia, and M. G. Paggi (2003)
Cancer Res. 63, 1430-1437
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Citron Kinase Is a Cell Cycle-dependent, Nuclear Protein Required for G2/M Transition of Hepatocytes.
H. Liu, F. Di Cunto, S. Imarisio, and L. M. Reid (2003)
J. Biol. Chem. 278, 2541-2548
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The centrosomal kinase Aurora-A/STK15 interacts with a putative tumor suppressor NM23-H1.
J. Du and G. J. Hannon (2002)
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epsilon -Tubulin Is an Essential Component of the Centriole.
S. K. Dutcher, N. S. Morrissette, A. M. Preble, C. Rackley, and J. Stanga (2002)
Mol. Biol. Cell 13, 3859-3869
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Distinct cell cycle-dependent roles for dynactin and dynein at centrosomes.
N. J. Quintyne and T. A. Schroer (2002)
J. Cell Biol. 159, 245-254
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The Mammalian Septin MSF Localizes with Microtubules and Is Required for Completion of Cytokinesis.
M. C. Surka, C. W. Tsang, and W. S. Trimble (2002)
Mol. Biol. Cell 13, 3532-3545
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Multiple centrosomes arise from tetraploidy checkpoint failure and mitotic centrosome clusters in p53 and RB pocket protein-compromised cells.
F. Borel, O. D. Lohez, F. B. Lacroix, and R. L. Margolis (2002)
PNAS 99, 9819-9824
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Cyclin G2 Associates with Protein Phosphatase 2A Catalytic and Regulatory B' Subunits in Active Complexes and Induces Nuclear Aberrations and a G1/S Phase Cell Cycle Arrest.
D. A. Bennin, A. S. A. Don, T. Brake, J. L. McKenzie, H. Rosenbaum, L. Ortiz, A. A. DePaoli-Roach, and M. C. Horne (2002)
J. Biol. Chem. 277, 27449-27467
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Disruption of Centrosome Structure, Chromosome Segregation, and Cytokinesis by Misexpression of Human Cdc14A Phosphatase.
B. K. Kaiser, Z. A. Zimmerman, H. Charbonneau, and P. K. Jackson (2002)
Mol. Biol. Cell 13, 2289-2300
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Cytokinesis in Eukaryotes.
D. A. Guertin, S. Trautmann, and D. McCollum (2002)
Microbiol. Mol. Biol. Rev. 66, 155-178
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Centrosome Reorientation in Wound-Edge Cells Is Cell Type Specific.
A.-M. C. Yvon, J. W. Walker, B. Danowski, C. Fagerstrom, A. Khodjakov, and P. Wadsworth (2002)
Mol. Biol. Cell 13, 1871-1880
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The Rho-associated protein kinase p160ROCK is required for centrosome positioning.
V. Chevrier, M. Piel, N. Collomb, Y. Saoudi, R. Frank, M. Paintrand, S. Narumiya, M. Bornens, and D. Job (2002)
J. Cell Biol. 157, 807-817
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Centrosome cohesion is regulated by a balance of kinase and phosphatase activities.
P. Meraldi and E. A. Nigg (2002)
J. Cell Sci. 114, 3749-3757
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R. Kuriyama, C. Gustus, Y. Terada, Y. Uetake, and J. Matuliene (2002)
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T. L. Megraw, S. Kilaru, F. R. Turner, and T. C. Kaufman (2002)
J. Cell Sci. 115, 4707-4718
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"It Takes Two to Tango": understanding how centrosome duplication is regulated throughout the cell cycle.
E. H. Hinchcliffe and G. Sluder (2001)
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Requirement of a Centrosomal Activity for Cell Cycle Progression Through G1 into S Phase.
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beta III Spectrin Binds to the Arp1 Subunit of Dynactin.
E. A. Holleran, L. A. Ligon, M. Tokito, M. C. Stankewich, J. S. Morrow, and E. L. F. Holzbaur (2001)
J. Biol. Chem. 276, 36598-36605
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CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis.
R. Kuriyama, C. Gustus, Y. Terada, Y. Uetake, and J. Matuliene (2002)
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   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)