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Science 16 February 2001:
Vol. 291. no. 5507, pp. 1298 - 1302
DOI: 10.1126/science.1057437
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Reports

A High-Resolution Radiation Hybrid Map of the Human Genome Draft Sequence

Michael Olivier,1 Amita Aggarwal,1 Jennifer Allen,1 Annalisa A. Almendras,1 Eva S. Bajorek,1 Ellen M. Beasley,1* Shannon D. Brady,1 Jannette M. Bushard,1 Valerie I. Bustos,1 Angela Chu,1 Tisha R. Chung,1 Anniek De Witte,1 Mirian E. Denys,1 Rakly Dominguez,1 Nicole Y. Fang,1 Brian D. Foster,1 Robert W. Freudenberg,1 David Hadley,1 Libby R. Hamilton,1 Tonya J. Jeffrey,1 Libusha Kelly,1 Laura Lazzeroni,1 Michelle R. Levy,1 Saskia C. Lewis,1 Xia Liu,1 Frederick J. Lopez,1 Brent Louie,1 Joseph P. Marquis,1 Robert A. Martinez,1 Margaret K. Matsuura,1 Nedda S. Misherghi,1 Jolanna A. Norton,1 Adam Olshen,1dagger Shanti M. Perkins,1 Amy J. Perou,1 Chris Piercy,1 Mark Piercy,1 Fawn Qin,1 Tim Reif,1 Kelly Sheppard,1 Vida Shokoohi,1 Geoff A. Smick,1 Wei-Lin Sun,1 Elizabeth A. Stewart,1ddagger J. Fernando , Tejeda,1 Nguyet M. Tran,1 Tonatiuh Trejo,1 Nu T. Vo,1 Simon C. M. Yan,1 Deborah L. Zierten,1 Shaying Zhao,2 Ravi Sachidanandam,3 Barbara J. Trask,4 Richard M. Myers,1 David R. Cox1§

We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

1 Stanford Human Genome Center, Stanford University School of Medicine, 975 California Avenue, Palo Alto, CA 94304, USA.
2 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
3 Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
4 Division of Human Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA.
*   Present address: Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.

dagger    Present address: UCSF Comprehensive Cancer Center, 2340 Sutter Street, San Francisco, CA 94143, USA.

ddagger    Present address: Incyte Genomics Inc., 3160 Porter Drive, Palo Alto, CA 94304, USA.

§   To whom correspondence should be addressed. E-mail: cox{at}shgc.stanford.edu

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