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Science 9 February 2001:
Vol. 291. no. 5506, pp. 1051 - 1055
DOI: 10.1126/science.291.5506.1051
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Reports

Simultaneous Binding of PtdIns(4,5)P2 and Clathrin by AP180 in the Nucleation of Clathrin Lattices on Membranes

Marijn G. J. Ford, Barbara M. F. Pearse, Matthew K. Higgins, Yvonne Vallis, David J. Owen, Adele Gibson,* Colin R. Hopkins,* Philip R. Evans,dagger Harvey T. McMahondagger

Adaptor protein 180 (AP180) and its homolog, clathrin assembly lymphoid myeloid leukemia protein (CALM), are closely related proteins that play important roles in clathrin-mediated endocytosis. Here, we present the structure of the NH2-terminal domain of CALM bound to phosphatidylinositol-4,5- bisphosphate [PtdIns(4,5)P2] via a lysine-rich motif. This motif is found in other proteins predicted to have domains of similar structure (for example, Huntingtin interacting protein 1). The structure is in part similar to the epsin NH2-terminal (ENTH) domain, but epsin lacks the PtdIns(4,5)P2-binding site. Because AP180 could bind to PtdIns(4,5)P2 and clathrin simultaneously, it may serve to tether clathrin to the membrane. This was shown by using purified components and a budding assay on preformed lipid monolayers. In the presence of AP180, clathrin lattices formed on the monolayer. When AP2 was also present, coated pits were formed.

Medical Research Council (MRC) Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK.
*   MRC Laboratory of Molecular and Cell Biology, University College London, Gower Street, London, WC1E 6BT, UK.

dagger    To whom correspondence should be addressed. E-mail: pre{at}mrc-lmb.cam.ac.uk, hmm{at}mrc-lmb.cam.ac.uk

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J. Physiol. 553, 345-355
   Abstract »    Full Text »    PDF »
Effect of Clathrin Heavy Chain- and {alpha}-Adaptin-specific Small Inhibitory RNAs on Endocytic Accessory Proteins and Receptor Trafficking in HeLa Cells.
L. Hinrichsen, J. Harborth, L. Andrees, K. Weber, and E. J. Ungewickell (2003)
J. Biol. Chem. 278, 45160-45170
   Abstract »    Full Text »    PDF »
Potential role for a novel AP180-related protein during endocytosis in MDCK cells.
L. Kusner and C. Carlin (2003)
Am J Physiol Cell Physiol 285, C995-C1008
   Abstract »    Full Text »    PDF »
Recycling of Raft-associated Prohormone Sorting Receptor Carboxypeptidase E Requires Interaction with ARF6.
I. Arnaoutova, C. L. Jackson, O. S. Al-Awar, J. G. Donaldson, and Y. P. Loh (2003)
Mol. Biol. Cell 14, 4448-4457
   Abstract »    Full Text »    PDF »
Sorting it out: AP-2 and alternate clathrin adaptors in endocytic cargo selection.
L. M. Traub (2003)
J. Cell Biol. 163, 203-208
   Abstract »    Full Text »    PDF »
Clathrin-mediated endocytosis in AP-2-depleted cells.
A. Motley, N. A. Bright, M. N.J. Seaman, and M. S. Robinson (2003)
J. Cell Biol. 162, 909-918
   Abstract »    Full Text »    PDF »
Phosphatidylinositol phosphate 5-kinase I{beta} recruits AP-2 to the plasma membrane and regulates rates of constitutive endocytosis.
D. Padron, Y. J. Wang, M. Yamamoto, H. Yin, and M. G. Roth (2003)
J. Cell Biol. 162, 693-701
   Abstract »    Full Text »    PDF »
A Role for Epsin N-terminal Homology/AP180 N-terminal Homology (ENTH/ANTH) Domains in Tubulin Binding.
N. K. Hussain, M. Yamabhai, A. L. Bhakar, M. Metzler, S. S. G. Ferguson, M. R. Hayden, P. S. McPherson, and B. K. Kay (2003)
J. Biol. Chem. 278, 28823-28830
   Abstract »    Full Text »    PDF »
Protein Kinase C Controls Microtubule-based Traffic but Not Proteasomal Degradation of c-Met.
S. Kermorgant, D. Zicha, and P. J. Parker (2003)
J. Biol. Chem. 278, 28921-28929
   Abstract »    Full Text »    PDF »
Contrasting Membrane Interaction Mechanisms of AP180 N-terminal Homology (ANTH) and Epsin N-terminal Homology (ENTH) Domains.
R. V. Stahelin, F. Long, B. J. Peter, D. Murray, P. De Camilli, H. T. McMahon, and W. Cho (2003)
J. Biol. Chem. 278, 28993-28999
   Abstract »    Full Text »    PDF »
Distinct Mechanisms of Agonist-induced Endocytosis for Human Chemokine Receptors CCR5 and CXCR4.
S. Venkatesan, J. J. Rose, R. Lodge, P. M. Murphy, and J. F. Foley (2003)
Mol. Biol. Cell 14, 3305-3324
   Abstract »    Full Text »    PDF »
Mutations in the clathrin-assembly gene Picalm are responsible for the hematopoietic and iron metabolism abnormalities in fit1 mice.
M. L. Klebig, M. D. Wall, M. D. Potter, E. L. Rowe, D. A. Carpenter, and E. M. Rinchik (2003)
PNAS 100, 8360-8365
   Abstract »    Full Text »    PDF »
ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type I{gamma}.
M. Krauss, M. Kinuta, M. R. Wenk, P. De Camilli, K. Takei, and V. Haucke (2003)
J. Cell Biol. 162, 113-124
   Abstract »    Full Text »    PDF »
Surface Expression of Inward Rectifier Potassium Channels Is Controlled by Selective Golgi Export.
C. Stockklausner and N. Klocker (2003)
J. Biol. Chem. 278, 17000-17005
   Abstract »    Full Text »    PDF »
Human Rhinovirus Type 2 Is Internalized by Clathrin-Mediated Endocytosis.
L. Snyers, H. Zwickl, and D. Blaas (2003)
J. Virol. 77, 5360-5369
   Abstract »    Full Text »    PDF »


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