Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Science Signaling - Call For Papers

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Originally published in Science Express on 4 January 2001
Science 19 January 2001:
Vol. 291. no. 5503, pp. 498 - 501
DOI: 10.1126/science.1057766
Prev | Table of Contents | Next

Reports

Crystal Structure of an Initiation Factor Bound to the 30S Ribosomal Subunit

Andrew P. Carter,1 William M. Clemons Jr.,1 Ditlev E. Brodersen,1 Robert J. Morgan-Warren,1 Thomas Hartsch,2 Brian T. Wimberly,1 V. Ramakrishnan1*

Initiation of translation at the correct position on messenger RNA is essential for accurate protein synthesis. In prokaryotes, this process requires three initiation factors: IF1, IF2, and IF3. Here we report the crystal structure of a complex of IF1 and the 30S ribosomal subunit. Binding of IF1 occludes the ribosomal A site and flips out the functionally important bases A1492 and A1493 from helix 44 of 16S RNA, burying them in pockets in IF1. The binding of IF1 causes long-range changes in the conformation of H44 and leads to movement of the domains of 30S with respect to each other. The structure explains how localized changes at the ribosomal A site lead to global alterations in the conformation of the 30S subunit.

1 Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
2 Göttingen Genomics Laboratory, Institut für Mikrobiologie und Genetic, Georg-August-Universität Göttingen, Grisebachstr. 8, D-37077 Göttingen, Germany.
*   To whom correspondence should be addressed. E-mail: ramak{at}mrc-lmb.cam.ac.uk

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Genetic Interaction Screens with Ordered Overexpression and Deletion Clone Sets Implicate the Escherichia coli GTPase YjeQ in Late Ribosome Biogenesis.
T. L. Campbell and E. D. Brown (2008)
J. Bacteriol. 190, 2537-2545
   Abstract »    Full Text »    PDF »
Factor requirements for translation initiation on the Simian picornavirus internal ribosomal entry site.
S. de Breyne, Y. Yu, T. V. Pestova, and C. U.T. Hellen (2008)
RNA 14, 367-380
   Abstract »    Full Text »    PDF »
Inaugural Article: The process of mRNA-tRNA translocation.
J. Frank, H. Gao, J. Sengupta, N. Gao, and D. J. Taylor (2007)
PNAS 104, 19671-19678
   Abstract »    Full Text »    PDF »
Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association.
V. G. KOLUPAEVA, A. UNBEHAUN, I. B. LOMAKIN, C. U.T. HELLEN, and T. V. PESTOVA (2005)
RNA 11, 470-486
   Abstract »    Full Text »    PDF »
Initiation of Protein Synthesis in Bacteria.
B. S. Laursen, H. P. Sorensen, K. K. Mortensen, and H. U. Sperling-Petersen (2005)
Microbiol. Mol. Biol. Rev. 69, 101-123
   Abstract »    Full Text »    PDF »
Release of initiation factors from 48S complexes during ribosomal subunit joining and the link between establishment of codon-anticodon base-pairing and hydrolysis of eIF2-bound GTP.
A. Unbehaun, S. I. Borukhov, C. U.T. Hellen, and T. V. Pestova (2004)
Genes & Dev. 18, 3078-3093
   Abstract »    Full Text »    PDF »
Photolabile anticodon stem-loop analogs of tRNAPhe as probes of ribosomal structure and structural fluctuation at the decoding center.
Z. DRUZINA and B. S. COOPERMAN (2004)
RNA 10, 1550-1562
   Abstract »    Full Text »    PDF »
Substrate Binding Analysis of the 23S rRNA Methyltransferase RrmJ.
J. Hager, B. L. Staker, and U. Jakob (2004)
J. Bacteriol. 186, 6634-6642
   Abstract »    Full Text »    PDF »
Position of eukaryotic initiation factor eIF1 on the 40S ribosomal subunit determined by directed hydroxyl radical probing.
I. B. Lomakin, V. G. Kolupaeva, A. Marintchev, G. Wagner, and T. V. Pestova (2003)
Genes & Dev. 17, 2786-2797
   Abstract »    Full Text »    PDF »
Ribosomal localization of translation initiation factor IF2.
S. MARZI, W. KNIGHT, L. BRANDI, E. CASERTA, N. SOBOLEVA, W. E. HILL, C. O. GUALERZI, and J. S. LODMELL (2003)
RNA 9, 958-969
   Abstract »    Full Text »    PDF »
The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo.
L. Valasek, A. A. Mathew, B.-S. Shin, K. H. Nielsen, B. Szamecz, and A. G. Hinnebusch (2003)
Genes & Dev. 17, 786-799
   Abstract »    Full Text »    PDF »
Leaderless mRNAs Bind 70S Ribosomes More Strongly than 30S Ribosomal Subunits in Escherichia coli.
S. M. O'Donnell and G. R. Janssen (2002)
J. Bacteriol. 184, 6730-6733
   Abstract »    Full Text »    PDF »
Oligonucleotide directed misfolding of RNA inhibits Candida albicans group I intron splicing.
J. L. Childs, M. D. Disney, and D. H. Turner (2002)
PNAS 99, 11091-11096
   Abstract »    Full Text »    PDF »
Complementation of Cold Shock Proteins by Translation Initiation Factor IF1 In Vivo.
M. H. W. Weber, C. L. Beckering, and M. A. Marahiel (2001)
J. Bacteriol. 183, 7381-7386
   Abstract »    Full Text »    PDF »
Structure and dynamics of translation initiation factor aIF-1A from the archaeon Methanococcus jannaschii determined by NMR spectroscopy.
W. Li and D. W. Hoffman (2001)
Protein Sci. 10, 2426-2438
   Abstract »    Full Text »    PDF »
AdoMet-dependent methylation, DNA methyltransferases and base flipping.
X. Cheng and R. J. Roberts (2001)
Nucleic Acids Res. 29, 3784-3795
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)