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Science 12 January 2001:
Vol. 291. no. 5502, pp. 303 - 305
DOI: 10.1126/science.291.5502.303
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Reports

A Ribonucleotide Reductase Homolog of Cytomegalovirus and Endothelial Cell Tropism

Wolfram Brune,12* Carine Ménard,2 Jürgen Heesemann,2 Ulrich H. Koszinowski2

Human cytomegalovirus infects vascular tissues and has been associated with atherogenesis and coronary restenosis. Although established laboratory strains of human cytomegalovirus have lost the ability to grow on vascular endothelial cells, laboratory strains of murine cytomegalovirus retain this ability. With the use of a forward-genetic procedure involving random transposon mutagenesis and rapid phenotypic screening, we identified a murine cytomegalovirus gene governing endothelial cell tropism. This gene, M45, shares sequence homology to ribonucleotide reductase genes. Endothelial cells infected with M45-mutant viruses rapidly undergo apoptosis, suggesting that a viral strategy to evade destruction by cellular apoptosis is indispensable for viral growth in endothelial cells.

1 Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
2 Genzentrum and Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität München, Pettenkoferstrasse 9a, 80336 Munich, Germany.
*   To whom correspondence should be addressed. E-mail: wbrune{at}princeton.edu

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