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Science 5 January 2001:
Vol. 291. no. 5501, pp. 121 - 125
DOI: 10.1126/science.291.5501.121
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Reports

Active Disruption of an RNA-Protein Interaction by a DExH/D RNA Helicase

Eckhard Jankowsky,1 Christian H. Gross,2 Stewart Shuman,2 Anna Marie Pyle13*

All aspects of cellular RNA metabolism and the replication of many viruses require DExH/D proteins that manipulate RNA in a manner that requires nucleoside triphosphates. Although DExH/D proteins have been shown to unwind purified RNA duplexes, most RNA molecules in the cellular environment are complexed with proteins. It has therefore been speculated that DExH/D proteins may also affect RNA-protein interactions. We demonstrate that the DExH protein NPH-II from vaccinia virus can displace the protein U1A from RNA in an active adenosine triphosphate-dependent fashion. NPH-II increases the rate of U1A dissociation by more than three orders of magnitude while retaining helicase processivity. This indicates that DExH/D proteins can effectively catalyze protein displacement from RNA and thereby participate in the structural reorganization of ribonucleoprotein assemblies.

1 Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
2 Sloan Kettering Institute, New York, NY 10021, USA.
3 Howard Hughes Medical Institute, Boston, MA 02115, USA.
*   To whom correspondence should be addressed. E-mail: amp11{at}columbia.edu

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