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Science 1 December 2000:
Vol. 290. no. 5497, pp. 1768 - 1771
DOI: 10.1126/science.290.5497.1768
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Reports

Down-Regulation of the Macrophage Lineage Through Interaction with OX2 (CD200)

Robert M. Hoek,1 Sigrid R. Ruuls,1 Craig A. Murphy,1 Gavin J. Wright,2 Ruth Goddard,2 Sandra M. Zurawski,1 Bianca Blom,1 Margit E. Homola,1 Wolfgang J. Streit,3 Marion H. Brown,2 A. Neil Barclay,2 Jonathon D. Sedgwick1*

OX2 (CD200) is a broadly expressed membrane glycoprotein, shown here to be important for regulation of the macrophage lineage. In mice lacking CD200, macrophage lineage cells, including brain microglia, exhibited an activated phenotype and were more numerous. Upon facial nerve transection, damaged CD200-deficient neurons elicited an accelerated microglial response. Lack of CD200 resulted in a more rapid onset of experimental autoimmune encephalomyelitis (EAE). Outside the brain, disruption of CD200-CD200 receptor interaction precipitated susceptibility to collagen-induced arthritis (CIA) in mice normally resistant to this disease. Thus, in diverse tissues OX2 delivers an inhibitory signal for the macrophage lineage.

1 DNAX Research Institute of Molecular and Cellular Biology, 901 California Avenue, Palo Alto, CA 94304, USA.
2 Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK.
3 Department of Neuroscience, College of Medicine and Brain Institute, University of Florida, Gainsville, FL 32610, USA.
*   To whom correspondence should be addressed. E-mail: jon.sedgwick{at}dnax.org

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