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Science 10 November 2000:
Vol. 290. no. 5494, pp. 1159 - 1162
DOI: 10.1126/science.290.5494.1159
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Reports

Transgene and Transposon Silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA Helicase

Dancia Wu-Scharf, Byeong-ryool Jeong, Chaomei Zhang, Heriberto Cerutti*

The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families. Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes.

School of Biological Sciences and Plant Science Initiative, University of Nebraska-Lincoln, E211 Beadle Center, Post Office Box 880666, Lincoln, NE 68588, USA.
*   To whom correspondence should be addressed. E-mail: hcerutti1{at}unl.edu

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