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Science 3 November 2000:
Vol. 290. no. 5493, pp. 995 - 997
DOI: 10.1126/science.290.5493.995
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Reports

Role of Bacillus subtilis SpoIIIE in DNA Transport Across the Mother Cell-Prespore Division Septum

Jonathan Bath,1 Ling Juan Wu,2 Jeffery Errington,2 James C. Wang1*

The SpoIIIE protein of Bacillus subtilis is required for chromosome segregation during spore formation. The COOH-terminal cytoplasmic part of SpoIIIE was shown to be a DNA-dependent adenosine triphosphatase (ATPase) capable of tracking along DNA in the presence of ATP, and the NH2-terminal part of the protein was found to mediate its localization to the division septum. Thus, during sporulation, SpoIIIE appears to act as a DNA pump that actively moves one of the replicated pair of chromosomes into the prespore. The presence of SpoIIIE homologs in a broad range of bacteria suggests that this mechanism for active transport of DNA may be widespread.

1 Department of Molecular and Cellular Biology, Harvard University, MA 02138, USA.
2 Sir William Dunn School of Pathology, Oxford University, Oxford OX1 3RE, UK.
*   To whom correspondence should be addressed. E-mail: jcwang{at}fas.harvard.edu

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