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A PEST-Like Sequence in Listeriolysin O Essential for Listeria monocytogenes Pathogenicity
Amy L. Decatur,1and Daniel A. Portnoy12*
Establishment and maintenance of an intracellular niche are
critical to the success of an intracellular pathogen. Here, thepore-forming protein listeriolysin O (LLO), secreted by Listeriamonocytogenes, was shown to contain a PEST-like sequence (P, Pro;E, Glu; S, Ser; T, Thr) that is essential for the virulence andintracellular compartmentalization of this pathogen. Mutants lackingthe PEST-like sequence entered the host cytosol but subsequentlypermeabilized and killed the host cell. LLO lacking the PEST-likesequence accumulated in the host-cell cytosol, suggesting thatthis
sequence targets LLO for degradation. Transfer of the sequenceto
perfringolysin O transformed this toxic cytolysin into a nontoxicderivative that facilitated intracellular growth.
1 Department of Molecular and Cell Biology,
2 Division of Infectious Diseases, School of Public
Health, University of California, Berkeley, CA 94720, USA.
*
To whom correspondence should be addressed. E-mail:
portnoy{at}uclink4.berkeley.edu
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