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Science 3 November 2000:
Vol. 290. no. 5493, pp. 992 - 995
DOI: 10.1126/science.290.5493.992
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Reports

A PEST-Like Sequence in Listeriolysin O Essential for Listeria monocytogenes Pathogenicity

Amy L. Decatur,1 and Daniel A. Portnoy12*

Establishment and maintenance of an intracellular niche are critical to the success of an intracellular pathogen. Here, the pore-forming protein listeriolysin O (LLO), secreted by Listeria monocytogenes, was shown to contain a PEST-like sequence (P, Pro; E, Glu; S, Ser; T, Thr) that is essential for the virulence and intracellular compartmentalization of this pathogen. Mutants lacking the PEST-like sequence entered the host cytosol but subsequently permeabilized and killed the host cell. LLO lacking the PEST-like sequence accumulated in the host-cell cytosol, suggesting that this sequence targets LLO for degradation. Transfer of the sequence to perfringolysin O transformed this toxic cytolysin into a nontoxic derivative that facilitated intracellular growth.

1 Department of Molecular and Cell Biology,
2 Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720, USA.
*   To whom correspondence should be addressed. E-mail: portnoy{at}uclink4.berkeley.edu

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