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Science 3 November 2000:
Vol. 290. no. 5493, pp. 982 - 984
DOI: 10.1126/science.290.5493.982
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Reports

A Basal Transcription Factor That Activates or Represses Transcription

Patricia J. Willy,1 Ryuji Kobayashi,2 James T. Kadonaga1*

We have identified an activity that is required for transcription of downstream promoter element (DPE)-containing core promoters in vitro. The purified factor was found to be the Drosophila homolog of the transcriptional repressor known as NC2 or Dr1-Drap1. Purified recombinant dNC2 activates DPE-driven promoters and represses TATA-driven promoters. A mutant version of dNC2 can activate DPE promoters but is unable to repress TATA promoters. Thus, the activation and repression functions are distinct. These studies reveal that NC2 (Dr1-Drap1) is a bifunctional basal transcription factor that differentially regulates gene transcription through DPE or TATA box motifs.

1 Section of Molecular Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0347, USA.
2 Cold Spring Harbor Laboratory, Post Office Box 100, Cold Spring Harbor, NY 11724, USA.
*   To whom correspondence should be addressed. E-mail: jkadonaga{at}ucsd.edu

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