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Science 20 October 2000:
Vol. 290. no. 5491, pp. 527 - 530
DOI: 10.1126/science.290.5491.527
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Reports

CD95/CD95 Ligand Interactions on Epithelial Cells in Host Defense to Pseudomonas aeruginosa

Heike Grassmé,1 Susanne Kirschnek,1 Joachim Riethmueller,1 Andrea Riehle,1 Gabriele von Kürthy,2 Florian Lang,1 Michael Weller,2 Erich Gulbins1*dagger

Pseudomonas aeruginosa causes severe infections, particularly of the lung, that are life threatening. Here, we show that P. aeruginosa infection induces apoptosis of lung epithelial cells by activation of the endogenous CD95/CD95 ligand system. Deficiency of CD95 or CD95 ligand on epithelial cells prevented apoptosis of lung epithelial cells in vivo as well as in vitro. The importance of CD95/CD95 ligand-mediated lung epithelial cell apoptosis was demonstrated by the rapid development of sepsis in CD95- or CD95 ligand-deficient mice, but not in normal mice, after P. aeruginosa infection.

1 Department of Physiology, University of Tuebingen, Gmelinstrasse 5, 72076 Tuebingen, Germany.
2 Department of Neurology, University of Tuebingen, Hoppe-Seyler Strasse 3, 72076 Tuebingen, Germany.
*   Present address: Department of Immunology, St. Jude Children's Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA.

dagger    To whom correspondence should be addressed. E-mail: erich.gulbins{at}stjude.org

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