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Anton Simeonov,1Masayuki Matsushita,1Eric A. Juban,2Elizabeth H. Z. Thompson,3Timothy Z. Hoffman,1Albert E. Beuscher IV12Matthew J. Taylor,1Peter Wirsching,1Wolfgang Rettig,4James K. McCusker,2Raymond C. Stevens,3David P. Millar,3Peter G. Schultz,1Richard A. Lerner,1Kim D. Janda1
The forte of catalytic antibodies has resided in the control of the
ground-state reaction coordinate. A principle and methodare now
described in which antibodies can direct the outcome ofphotophysical
and photochemical events that take place on excited-statepotential
energy surfaces. The key component is a chemically reactiveoptical
sensor that provides a direct report of the dynamic interplaybetween
protein and ligand at the active site. To illustrate theconcept, we
used a trans-stilbene hapten to elicit a panel ofmonoclonal
antibodies that displayed a range of fluorescent spectralbehavior when
bound to a trans-stilbene substrate. Several antibodiesyielded a blue fluorescence indicative of an excited-state complexor
"exciplex" between trans-stilbene and the antibody. The
antibodiescontrolled the isomerization coordinate of
trans-stilbene anddynamically coupled this manifold with an
active-site residue.A step was taken toward the use of antibody-based
photochemicalsensors for diagnostic and clinical applications.
1 Department of Chemistry, The Scripps Research
Institute and the Skaggs Institute for Chemical Biology, 10550 North
Torrey Pines Road, La Jolla, CA 92037, USA.
2 Department of Chemistry, University of California
Berkeley, Berkeley, CA 94720-1460, USA.
3 Department of Molecular Biology, The Scripps
Research Institute, 10555 North Torrey Pines Road, La Jolla, CA 92037, USA.
4 Institute of Physical and Theoretical
Chemistry, Humboldt-University Berlin, Bunsenstrasse 1 D-10117
Berlin, Germany.
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In Science Magazine
PERSPECTIVES
John I. Brauman (13 October 2000) Science290 (5490), 286.
[DOI: 10.1126/science.290.5490.286] |Summary »|Full Text »
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Deeply Inverted Electron-Hole Recombination in a Luminescent Antibody-Stilbene Complex.
E. W. Debler, G. F. Kaufmann, M. M. Meijler, A. Heine, J. M. Mee, G. Pljevaljcic, A. J. Di Bilio, P. G. Schultz, D. P. Millar, K. D. Janda, et al. (2008)
Science
319, 1232-1235
|Abstract »|Full Text »|PDF »
