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Science 6 October 2000:
Vol. 290. no. 5489, pp. 142 - 144
DOI: 10.1126/science.290.5489.142
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Reports

A Calmodulin-Related Protein That Suppresses Posttranscriptional Gene Silencing in Plants

Radhamani Anandalakshmi,1* Rajendra Marathe,1* Xin Ge,1 J. M. Herr Jr.,1 Christopher Mau,2 Allison Mallory,1 Gail Pruss,1 Lewis Bowman,1 Vicki B. Vance1dagger

Posttranscriptional gene silencing (PTGS) is an ancient eukaryotic regulatory mechanism in which a particular RNA sequence is targeted and destroyed. The helper component-proteinase (HC-Pro) of plant potyviruses suppresses PTGS in plants. Using a yeast two-hybrid system, we identified a calmodulin-related protein (termed rgs-CaM) that interacts with HC-Pro. Here we report that rgs-CaM, like HC-Pro itself, suppresses gene silencing. Our work is the first report identifying a cellular suppressor of PTGS.

1 Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA.
2 CEPRAP, University of California, Davis, CA 95616, USA.
*   These authors contributed equally to this report.

dagger    To whom correspondence should be addressed. E-mail: vance{at}biol.sc.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)