Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 29 September 2000:
Vol. 289. no. 5488, pp. 2360 - 2362
DOI: 10.1126/science.289.5488.2360
Prev | Table of Contents | Next

Reports

Active Remodeling of Somatic Nuclei in Egg Cytoplasm by the Nucleosomal ATPase ISWI

Nobuaki Kikyo,1 Paul A. Wade,1 Dmitry Guschin,2 Hui Ge,1 Alan P. Wolffe2*

Cloning by the transplantation of somatic nuclei into unfertilized eggs requires a dramatic remodeling of chromosomal architecture. Many proteins are specifically lost from nuclei, and others are taken up from the egg cytoplasm. Recreating this exchange in vitro, we identified the chromatin-remodeling nucleosomal adenosine triphosphatase (ATPase) ISWI as a key molecule in this process. ISWI actively erases the TATA binding protein from association with the nuclear matrix. Defining the biochemistry of global nuclear remodeling may facilitate the efficiency of cloning and other dedifferentiation events that establish new stem cell lineages.

1 Laboratory of Molecular Embryology, Building 18T, Room 106, National Institutes of Health, Bethesda, MD 20892, USA.
2 Sangamo BioSciences, Point Richmond Tech Center, 501 Canal Boulevard, Suite A100, Richmond, CA 94804, USA.
*   To whom correspondence should be addressed. E-mail: awolffe{at}sangamo.com

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cell-Free Extracts from Mammalian Oocytes Partially Induce Nuclear Reprogramming in Somatic Cells.
K. Miyamoto, T. Tsukiyama, Y. Yang, N. Li, N. Minami, M. Yamada, and H. Imai (2009)
Biol Reprod 80, 935-943
   Abstract »    Full Text »    PDF »
Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization.
D. Luo, W. Hu, S. Chen, Y. Xiao, Y. Sun, and Z. Zhu (2009)
Biol Reprod 80, 674-684
   Abstract »    Full Text »    PDF »
Epigenetic regulation of stem cell fate.
V. V. Lunyak and M. G. Rosenfeld (2008)
Hum. Mol. Genet. 17, R28-R36
   Abstract »    Full Text »    PDF »
Identification of Genes Aberrantly Expressed in Mouse Embryonic Stem Cell-Cloned Blastocysts.
Y. Jincho, Y. Sotomaru, M. Kawahara, Y. Ono, H. Ogawa, Y. Obata, and T. Kono (2008)
Biol Reprod 78, 568-576
   Abstract »    Full Text »    PDF »
Reprogramming somatic cells into stem cells..
R. Alberio, K. H Campbell, and A. D Johnson (2006)
Reproduction 132, 709-720
   Abstract »    Full Text »    PDF »
Dynamic Regulation of Histone Modifications in Xenopus Oocytes through Histone Exchange..
M. D. Stewart, J. Sommerville, and J. Wong (2006)
Mol. Cell. Biol. 26, 6890-6901
   Abstract »    Full Text »    PDF »
Epigenetic characteristics of cloned and in vitro-fertilized swamp buffalo (Bubalus bubalis) embryos.
T. Suteevun, R. Parnpai, S. L. Smith, C-C. Chang, S. Muenthaisong, and X. C. Tian (2006)
J Anim Sci 84, 2065-2071
   Abstract »    Full Text »    PDF »
Nuclear transcription is essential for specification of mammalian replication origins..
D. S. Dimitrova (2006)
Genes Cells 11, 829-844
   Abstract »    Full Text »    PDF »
Maternal BRG1 regulates zygotic genome activation in the mouse..
S. J. Bultman, T. C. Gebuhr, H. Pan, P. Svoboda, R. M. Schultz, and T. Magnuson (2006)
Genes & Dev. 20, 1744-1754
   Abstract »    Full Text »    PDF »
Acquisition and extinction of gene expression programs are separable events in heterokaryon reprogramming.
R. Terranova, C. F. Pereira, C. Du Roure, M. Merkenschlager, and A. G. Fisher (2006)
J. Cell Sci. 119, 2065-2072
   Abstract »    Full Text »    PDF »
Chromatin loop domain organization within the 4q35 locus in facioscapulohumeral dystrophy patients versus normal human myoblasts.
A. Petrov, I. Pirozhkova, G. Carnac, D. Laoudj, M. Lipinski, and Y. S. Vassetzky (2006)
PNAS 103, 6982-6987
   Abstract »    Full Text »    PDF »
Requirement of the Protein B23 for Nucleolar Disassembly Induced by the FRGY2a Family Proteins.
K. Gonda, J. Wudel, D. Nelson, N. Katoku-Kikyo, P. Reed, H. Tamada, and N. Kikyo (2006)
J. Biol. Chem. 281, 8153-8160
   Abstract »    Full Text »    PDF »
Chromatin Decondensation and Nuclear Reprogramming by Nucleoplasmin.
H. Tamada, N. V. Thuan, P. Reed, D. Nelson, N. Katoku-Kikyo, J. Wudel, T. Wakayama, and N. Kikyo (2006)
Mol. Cell. Biol. 26, 1259-1271
   Abstract »    Full Text »    PDF »
Human Embryonic Stem Cells Reprogram Myeloid Precursors Following Cell-Cell Fusion.
J. Yu, M. A. Vodyanik, P. He, I. I. Slukvin, and J. A. Thomson (2006)
Stem Cells 24, 168-176
   Abstract »    Full Text »    PDF »
Induction of Dedifferentiation, Genomewide Transcriptional Programming, and Epigenetic Reprogramming by Extracts of Carcinoma and Embryonic Stem Cells.
C. K. Taranger, A. Noer, A. L. Sorensen, A.-M. Hakelien, A. C. Boquest, and P. Collas (2005)
Mol. Biol. Cell 16, 5719-5735
   Abstract »    Full Text »    PDF »
Chromatin remodeling and histone modification in the conversion of oligodendrocyte precursors to neural stem cells.
T. Kondo and M. Raff (2004)
Genes & Dev. 18, 2963-2972
   Abstract »    Full Text »    PDF »
Identification of Porcine Oocyte Proteins That Are Associated with Somatic Cell Nuclei after Co-Incubation.
S. Novak, F. Paradis, C. Savard, K. Tremblay, and M.-A. Sirard (2004)
Biol Reprod 71, 1279-1289
   Abstract »    Full Text »    PDF »
Histone Fold Protein Dls1p Is Required for Isw2-Dependent Chromatin Remodeling In Vivo.
A. D. McConnell, M. E. Gelbart, and T. Tsukiyama (2004)
Mol. Cell. Biol. 24, 2605-2613
   Abstract »    Full Text »    PDF »
Cloned Calves from Chromatin Remodeled In Vitro.
E. J. Sullivan, S. Kasinathan, P. Kasinathan, J. M. Robl, and P. Collas (2004)
Biol Reprod 70, 146-153
   Abstract »    Full Text »    PDF »
Reprogramming of Bovine Somatic Cell Nuclei Is Not Directly Regulated by Maturation Promoting Factor or Mitogen-Activated Protein Kinase Activity.
T. Tani, Y. Kato, and Y. Tsunoda (2003)
Biol Reprod 69, 1890-1894
   Abstract »    Full Text »    PDF »
The first half-century of nuclear transplantation.
J. B. Gurdon and J. A. Byrne (2003)
PNAS 100, 8048-8052
   Abstract »    Full Text »    PDF »
Changes in histone acetylation during mouse oocyte meiosis.
J.-M. Kim, H. Liu, M. Tazaki, M. Nagata, and F. Aoki (2003)
J. Cell Biol. 162, 37-46
   Abstract »    Full Text »    PDF »
Analysis of the Mechanism for Chromatin Remodeling in Embryos Reconstructed by Somatic Nuclear Transfer.
J.-M. Kim, A. Ogura, M. Nagata, and F. Aoki (2002)
Biol Reprod 67, 760-766
   Abstract »    Full Text »    PDF »
Nuclei of Nonviable Ovine Somatic Cells Develop into Lambs after Nuclear Transplantation.
P. Loi, M. Clinton, B. Barboni, J. Fulka Jr., P. Cappai, R. Feil, R. M. Moor, and G. Ptak (2002)
Biol Reprod 67, 126-132
   Abstract »    Full Text »    PDF »
Nucleosome mobilization and positioning by ISWI-containing chromatin-remodeling factors.
G. Langst and P. B. Becker (2002)
J. Cell Sci. 114, 2561-2568
   Abstract »    Full Text »    PDF »
Conservation of methylation reprogramming in mammalian development: Aberrant reprogramming in cloned embryos.
W. Dean, F. Santos, M. Stojkovic, V. Zakhartchenko, J. Walter, E. Wolf, and W. Reik (2001)
PNAS 98, 13734-13738
   Abstract »    Full Text »    PDF »
Histone tails modulate nucleosome mobility and regulate ATP-dependent nucleosome sliding by NURF.
A. Hamiche, J.-G. Kang, C. Dennis, H. Xiao, and C. Wu (2001)
PNAS
   Abstract »    Full Text »    PDF »
Widespread Collaboration of Isw2 and Sin3-Rpd3 Chromatin Remodeling Complexes in Transcriptional Repression.
T. G. Fazzio, C. Kooperberg, J. P. Goldmark, C. Neal, R. Basom, J. Delrow, and T. Tsukiyama (2001)
Mol. Cell. Biol. 21, 6450-6460
   Abstract »    Full Text »    PDF »
Nuclear Cloning and Epigenetic Reprogramming of the Genome.
W. M. Rideout III, K. Eggan, and R. Jaenisch (2001)
Science 293, 1093-1098
   Abstract »    Full Text »    PDF »
In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p.
N. A. Kent, N. Karabetsou, P. K. Politis, and J. Mellor (2001)
Genes & Dev. 15, 619-626
   Abstract »    Full Text »
Two Phases of Chromatin Decondensation during Dedifferentiation of Plant Cells. DISTINCTION BETWEEN COMPETENCE FOR CELL FATE SWITCH AND A COMMITMENT FOR S PHASE.
J. Zhao, N. Morozova, L. Williams, L. Libs, Y. Avivi, and G. Grafi (2001)
J. Biol. Chem. 276, 22772-22778
   Abstract »    Full Text »    PDF »
Histone tails modulate nucleosome mobility and regulate ATP-dependent nucleosome sliding by NURF.
A. Hamiche, J.-G. Kang, C. Dennis, H. Xiao, and C. Wu (2001)
PNAS 98, 14316-14321
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)