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Science 29 September 2000:
Vol. 289. no. 5488, pp. 2338 - 2342
DOI: 10.1126/science.289.5488.2338
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Reports

Alteration of Stimulus-Specific Guard Cell Calcium Oscillations and Stomatal Closing in Arabidopsis det3 Mutant

Gethyn J. Allen,1* Sarah P. Chu,1dagger Karin Schumacher,2dagger ddagger Chad T. Shimazaki,1 Dionne Vafeados,2 Andrea Kemper,3 Scott D. Hawke,3 Gary Tallman,3 Roger Y. Tsien,4 Jeffrey F. Harper,5 Joanne Chory,2 Julian I. Schroeder1*

Cytosolic calcium oscillations control signaling in animal cells, whereas in plants their importance remains largely unknown. In wild-type Arabidopsis guard cells abscisic acid, oxidative stress, cold, and external calcium elicited cytosolic calcium oscillations of differing amplitudes and frequencies and induced stomatal closure. In guard cells of the V-ATPase mutant det3, external calcium and oxidative stress elicited prolonged calcium increases, which did not oscillate, and stomatal closure was abolished. Conversely, cold and abscisic acid elicited calcium oscillations in det3, and stomatal closure occurred normally. Moreover, in det3 guard cells, experimentally imposing external calcium-induced oscillations rescued stomatal closure. These data provide genetic evidence that stimulus-specific calcium oscillations are necessary for stomatal closure.

1 Cell and Developmental Biology Section, Division of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0116, USA.
2 Howard Hughes Medical Institute and Plant Biology Laboratory, Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037-1099, USA.
3 Department of Biology, Willamette University, 900 State Street, Salem, OR 97301-3931, USA.
4 Department of Pharmacology and Chemistry and Biochemistry, Howard Hughes Medical Institute and Division of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093-0647, USA.
5 Department of Cell Biology, Scripps Research Institute, BCC283, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
*   To whom correspondence should be addressed. E-mail: gallen{at}biomail.ucsd.edu, julian{at}biomail.ucsd.edu

dagger    These authors contributed equally to this work.

ddagger    Present address: ZMBP-Pflanzenphysiologie, Universitaet Tuebingen, Auf der Morgenstelle 1, 72076 Tuebingen, Germany.

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