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Science 15 September 2000:
Vol. 289. no. 5486, pp. 1931 - 1933
DOI: 10.1126/science.289.5486.1931
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Reports

Suppression of Mutations in Mitochondrial DNA by tRNAs Imported from the Cytoplasm

O. A. Kolesnikova,12* N. S. Entelis,12* H. Mireau,3dagger T. D. Fox,3 R. P. Martin,1 I. A. Tarassov1ddagger

Mitochondrial import of a cytoplasmic transfer RNA (tRNA) in yeast requires the preprotein import machinery and cytosolic factors. We investigated whether the tRNA import pathway can be used to correct respiratory deficiencies due to mutations in the mitochondrial DNA and whether this system can be transferred into human cells. We show that cytoplasmic tRNAs with altered aminoacylation identity can be specifically targeted to the mitochondria and participate in mitochondrial translation. We also show that human mitochondria, which do not normally import tRNAs, are able to internalize yeast tRNA derivatives in vitro and that this import requires an essential yeast import factor.

1 FRE 2168 du CNRS, Mécanismes Moléculaires de la Division Cellulaire et du Développement, 21 rue René Descartes, 67084 Strasbourg, France.
2 Department of Molecular Biology, Moscow State University, 119899 Moscow, Russia.
3 Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703, USA.
*   These authors contributed equally to this work.

dagger    Present address: INRA, route de Saint-Cyr, Versailles 78026, France.

ddagger    To whom correspondence should be addressed. E-mail: I.Tarassov{at}ibmc.u-strasbg.fr

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Science. ISSN 0036-8075 (print), 1095-9203 (online)