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Science 15 September 2000:
Vol. 289. no. 5486, pp. 1928 - 1930
DOI: 10.1126/science.289.5486.1928
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Reports

A Link Between RNA Interference and Nonsense-Mediated Decay in Caenorhabditis elegans

Mary Ellen Domeier,1 Daniel P. Morse,2 Scott W. Knight,2 Michael Portereiko,1 Brenda L. Bass,2 Susan E. Mango1*

Double-stranded RNA (dsRNA) inhibits expression of homologous genes by a process involving messenger RNA degradation. To gain insight into the mechanism of degradation, we examined how RNA interference is affected by mutations in the smg genes, which are required for nonsense-mediated decay. For three of six smg genes tested, mutations resulted in animals that were initially silenced by dsRNA but then recovered; wild-type animals remained silenced. The levels of target messenger RNAs were restored during recovery, and RNA editing and degradation of the dsRNA were identical to those of the wild type. We suggest that persistence of RNA interference relies on a subset of smg genes.

1 Huntsman Cancer Institute Center for Children and Department of Oncological Sciences, University of Utah, Salt Lake City, UT 84112, USA.
2 Department of Biochemistry and Howard Hughes Medical Institute, University of Utah, Salt Lake City, UT 84132, USA.
*   To whom correspondence should be addressed. E-mail: susan.mango{at}hci.utah.edu

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