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Science 18 August 2000:
Vol. 289. no. 5482, pp. 1188 - 1190
DOI: 10.1126/science.289.5482.1188
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Reports

Pig Cloning by Microinjection of Fetal Fibroblast Nuclei

Akira Onishi,1* Masaki Iwamoto,2 Tomiji Akita,1 Satoshi Mikawa,1 Kumiko Takeda,1 Takashi Awata,1 Hirohumi Hanada,1 Anthony C. F. Perry3

Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine (Sus scrofa) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis.

1 Department of Animal Breeding and Genetics, National Institute of Animal Industry, Tsukuba Norin Danchi, Ibaraki-ken 305-0901, Japan.
2 Basic Research Division, Prima Meatpackers, Tsuchiura-shi, Ibaraki-ken 300-0841, Japan.
3 Laboratory of Developmental Biology, The Rockefeller University, New York, NY 10021, USA.
*   To whom correspondence should be addressed. E-mail: onishi{at}niai.affrc.go.jp

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