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Science 14 July 2000:
Vol. 289. no. 5477, pp. 300 - 303
DOI: 10.1126/science.289.5477.300
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Reports

Requirement of the Spindle Checkpoint for Proper Chromosome Segregation in Budding Yeast Meiosis

Marion A. Shonn, 1* Robert McCarroll, 2 Andrew W. Murray 13*dagger

The spindle checkpoint was characterized in meiosis of budding yeast. In the absence of the checkpoint, the frequency of meiosis I missegregation increased with increasing chromosome length, reaching 19% for the longest chromosome. Meiosis I nondisjunction in spindle checkpoint mutants could be prevented by delaying the onset of anaphase. In a recombination-defective mutant (spo11Delta ), the checkpoint delays the biochemical events of anaphase I, suggesting that chromosomes that are attached to microtubules but are not under tension can activate the spindle checkpoint. Spindle checkpoint mutants reduce the accuracy of chromosome segregation in meiosis I much more than that in meiosis II, suggesting that checkpoint defects may contribute to Down syndrome.

1 Department of Biochemistry and
3 Department of Physiology, University of California, San Francisco, CA 94143-0444, USA.
2 Cereon Genomics, LLC, 45 Sidney Street, Cambridge, MA 02139, USA.
*   Present address: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.

dagger    To whom correspondence should be addressed. E-mail: amurray{at}mcb.harvard.edu

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