Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 14 July 2000:
Vol. 289. no. 5477, pp. 297 - 300
DOI: 10.1126/science.289.5477.297
Prev | Table of Contents | Next

Reports

An Inherited Functional Circadian Clock in Zebrafish Embryos

Franck Delaunay, 1 Christine Thisse, 2 Oriane Marchand, 1 Vincent Laudet, 1* Bernard Thisse 2

Circadian clocks are time-keeping systems found in most organisms. In zebrafish, expression of the clock gene Period3 (Per3) oscillates throughout embryogenesis in the central nervous system and the retina. Per3 rhythmic expression was free-running and was reset by light but not by the developmental delays caused by low temperature. The time of fertilization had no effect on Per3 expression. Per3 messenger RNA accumulates rhythmically in oocytes and persists in embryos. Our results establish that the circadian clock functions during early embryogenesis in zebrafish. Inheritance of maternal clock gene products suggests a mechanism of phase inheritance through ovogenesis.

1 Ecole Normale Supérieure, CNRS UMR 5665, 46 allée d'Italie, 69364 Lyon Cedex, France.
2 Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 163, 67404 Illkirch Cedex, Strasbourg, France.
*   To whom correspondence should be addressed. E-mail: vincent.laudet{at}ens-lyon.fr

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Circadian Clock Genes of Goldfish, Carassius auratus: cDNA Cloning and Rhythmic Expression of Period and Cryptochrome Transcripts in Retina, Liver, and Gut.
E. Velarde, R. Haque, P.M. Iuvone, C. Azpeleta, A.L. Alonso-Gomez, and M.J. Delgado (2009)
J Biol Rhythms 24, 104-113
   Abstract »    PDF »
Circadian Timekeeping during Early Arabidopsis Development.
P. A. Salome, Q. Xie, and C. R. McClung (2008)
Plant Physiology 147, 1110-1125
   Abstract »    Full Text »    PDF »
OTX5 Regulates Pineal Expression of the Zebrafish REV-ERB{alpha} through a New DNA Binding Site.
S.-i. Nishio, T. Kakizawa, G. Chatelain, G. Triqueneaux, F. Brunet, J. Rambaud, T. Lamonerie, and V. Laudet (2008)
Mol. Endocrinol. 22, 23-32
   Abstract »    Full Text »    PDF »
Matrix Metalloproteinase-13 Is Required for Zebra fish (Danio rerio) Development and Is a Target for Glucocorticoids.
J. M. Hillegass, C. M. Villano, K. R. Cooper, and L. A. White (2007)
Toxicol. Sci. 100, 168-179
   Abstract »    Full Text »    PDF »
Retinoic Acid Inhibits beta-Catenin through Suppression of Cox-2: A ROLE FOR TRUNCATED ADENOMATOUS POLYPOSIS COLI.
A. L. Eisinger, L. D. Nadauld, D. N. Shelton, S. M. Prescott, D. M. Stafforini, and D. A. Jones (2007)
J. Biol. Chem. 282, 29394-29400
   Abstract »    Full Text »    PDF »
Two differentially active alternative promoters control the expression of the zebrafish orphan nuclear receptor gene Rev-erb{alpha}.
T. Kakizawa, S.-i. Nishio, G. Triqueneaux, S. Bertrand, J. Rambaud, and V. Laudet (2007)
J. Mol. Endocrinol. 38, 555-568
   Abstract »    Full Text »    PDF »
Diversity of zebrafish peripheral oscillators revealed by luciferase reporting.
M. Kaneko, N. Hernandez-Borsetti, and G. M. Cahill (2006)
PNAS 103, 14614-14619
   Abstract »    Full Text »    PDF »
The Adenomatous Polyposis Coli Tumor Suppressor Gene Regulates Expression of Cyclooxygenase-2 by a Mechanism That Involves Retinoic Acid.
A. L. Eisinger, L. D. Nadauld, D. N. Shelton, P. W. Peterson, R. A. Phelps, S. Chidester, D. M. Stafforini, S. M. Prescott, and D. A. Jones (2006)
J. Biol. Chem. 281, 20474-20482
   Abstract »    Full Text »    PDF »
Starting the Zebrafish Pineal Circadian Clock with a Single Photic Transition.
R. Vuilleumier, L. Besseau, G. Boeuf, A. Piparelli, Y. Gothilf, W. G. Gehring, D. C. Klein, and J. Falcon (2006)
Endocrinology 147, 2273-2279
   Abstract »    Full Text »    PDF »
Circadian time-keeping during early stages of development..
L. Ziv and Y. Gothilf (2006)
PNAS 103, 4146-4151
   Abstract »    Full Text »    PDF »
The orphan receptor Rev-erb{alpha} gene is a target of the circadian clock pacemaker.
G. Triqueneaux, S. Thenot, T. Kakizawa, M. P Antoch, R. Safi, J. S Takahashi, F. Delaunay, and V. Laudet (2004)
J. Mol. Endocrinol. 33, 585-608
   Abstract »    Full Text »    PDF »
E-box function in a period gene repressed by light.
D. Vallone, S. B. Gondi, D. Whitmore, and N. S. Foulkes (2004)
PNAS 101, 4106-4111
   Abstract »    Full Text »    PDF »
The Days and Nights of Cancer Cells.
L. Canaple, T. Kakizawa, and V. Laudet (2003)
Cancer Res. 63, 7545-7552
   Abstract »    Full Text »    PDF »
Entraining Signals Initiate Behavioral Circadian Rhythmicity in Larval Zebrafish.
M. W. Hurd and G. M. Cahill (2002)
J Biol Rhythms 17, 307-314
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)