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Science 7 July 2000:
Vol. 289. no. 5476, pp. 123 - 127
DOI: 10.1126/science.289.5476.123
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Reports

Structure of the Cytoplasmic beta  Subunit--T1 Assembly of Voltage-Dependent K+ Channels

Jacqueline M. Gulbis, *dagger Ming Zhou, * Sabine Mann, Roderick MacKinnon ddagger

The structure of the cytoplasmic assembly of voltage-dependent K+ channels was solved by x-ray crystallography at 2.1 angstrom resolution. The assembly includes the cytoplasmic (T1) domain of the integral membrane alpha  subunit together with the oxidoreductase beta  subunit in a fourfold symmetric T14beta 4 complex. An electrophysiological assay showed that this complex is oriented with four T1 domains facing the transmembrane pore and four beta  subunits facing the cytoplasm. The transmembrane pore communicates with the cytoplasm through lateral, negatively charged openings above the T14beta 4 complex. The inactivation peptides of voltage-dependent K+ channels reach their site of action by entering these openings.

Howard Hughes Medical Institute and Laboratory of Molecular Neurobiology and Biophysics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
*   These authors contributed equally to this work.

dagger    Present address: The Biomolecular Research Institute, 343 Royal Parade, Parkville, Victoria, 3052, Australia.

ddagger    To whom correspondence should be addressed. E-mail: mackinn{at}rockvax.rockefeller.edu

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