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Science 23 June 2000:
Vol. 288. no. 5474, pp. 2215 - 2219
DOI: 10.1126/science.288.5474.2215
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Reports

Requirement of Mis6 Centromere Connector for Localizing a CENP-A-Like Protein in Fission Yeast

Kohta Takahashi, 123 Ee Sin Chen, 12 Mitsuhiro Yanagida 123*

Mammalian kinetochores contain the centromere-specific histone H3 variant CENP-A, whose incorporation into limited chromosomal regions may be important for centromere function and chromosome segregation during mitosis. However, regulation of CENP-A localization and its role have not been clear. Here we report that the fission yeast homolog SpCENP-A is essential for establishing centromere chromatin associated with equal chromosome segregation. SpCENP-A binding to the nonrepetitious inner centromeres depended on Mis6, an essential centromere connector protein acting during G1-S phase of the cell cycle. Mis6 is likely required for recruiting SpCENP-A to form proper connection of sister centromeres.

1 CREST Research Project, Japan Science and Technology Corporation.
2 Department of Biophysics, Graduate School of Science,
3 Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
*   To whom correspondence should be addressed. E-mail: yanagida{at}kozo.biophys.kyoto-u.ac.jp

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