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Science 9 June 2000:
Vol. 288. no. 5472, pp. 1832 - 1835
DOI: 10.1126/science.288.5472.1832
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Reports

mGluR1 in Cerebellar Purkinje Cells Essential for Long-Term Depression, Synapse Elimination, and Motor Coordination

Taeko Ichise, 1 Masanobu Kano, 23 Kouichi Hashimoto, 23 Dai Yanagihara, 245 Kazuki Nakao, 1 Ryuichi Shigemoto, 26 Motoya Katsuki, 12 Atsu Aiba 1*

Targeted deletion of metabotropic glutamate receptor-subtype 1 (mGluR1) gene can cause defects in development and function in the cerebellum. We introduced the mGluR1alpha transgene into mGluR1-null mutant [mGluR1 (-/-)] mice with a Purkinje cell (PC)-specific promoter. mGluR1-rescue mice showed normal cerebellar long-term depression and regression of multiple climbing fiber innervation, events significantly impaired in mGluR1 (-/-) mice. The impaired motor coordination was rescued by this transgene, in a dose-dependent manner. We propose that mGluR1 in PCs is a key molecule for normal synapse formation, synaptic plasticity, and motor control in the cerebellum.

1 Laboratory of DNA Biology and Embryo Engineering, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
2 Core Research for Evolutional Science and Technology (CREST),
3 Department of Physiology, School of Medicine, Kanazawa University, Kanazawa 920-8640, Japan.
4 Toyohashi University of Technology, Toyohashi 441-8580, Japan.
5 Brain Science Institute, RIKEN, Wako 351-0198, Japan.
6 Laboratory of Cerebral Structure, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, Japan.
*   To whom correspondence should be addressed: E-mail: aiba{at}ims.u-tokyo.ac.jp

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