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Science 2 June 2000:
Vol. 288. no. 5471, pp. 1653 - 1656
DOI: 10.1126/science.288.5471.1653
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Reports

Intracellular Parasitism by the Human Granulocytic Ehrlichiosis Bacterium Through the P-Selectin Ligand, PSGL-1

Michael J. Herron, 1 Curtis M. Nelson, 1 Janet Larson, 1 Karen R. Snapp, 2 Geoffrey S. Kansas, 2 Jesse L. Goodman 1*

Human granulocytic ehrlichiosis (HGE) is a febrile tick-borne illness caused by a recently discovered intracellular bacterium remarkable for its tropism for professionally phagocytic neutrophils. Monoclonal antibodies against the P-selectin binding domain of the leukocyte P-selectin glycoprotein ligand, PSGL-1, prevented HGE cell binding and infection, as did enzymatic digestion of PSGL-1. Furthermore, simultaneous neoexpression in nonsusceptible cells of complementary DNAs for both PSGL-1 and its modifying alpha -(1,3) fucosyltransferase, Fuc-TVII, allowed binding and infection by HGE. Thus, the HGE bacterium specifically bound to fucosylated leukocyte PSGL-1. Selectin mimicry is likely central to the organism's unique ability to target and infect neutrophils.

1 Division of Infectious Diseases, Department of Medicine, University of Minnesota School of Medicine, Minneapolis, MN 55455, USA.
2 Department of Microbiology-Immunology, Northwestern University School of Medicine, Chicago, IL 60611, USA.
*   To whom correspondence should be addressed. E mail: goodmanj{at}cber.fda.gov

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