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Science 26 May 2000:
Vol. 288. no. 5470, pp. 1422 - 1425
DOI: 10.1126/science.288.5470.1422
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Reports

Structure and Function of a Human TAFII250 Double Bromodomain Module

Raymond H. Jacobson, Andreas G. Ladurner, David S. King, Robert Tjian *

TFIID is a large multiprotein complex that initiates assembly of the transcription machinery. It is unclear how TFIID recognizes promoters in vivo when templates are nucleosome-bound. Here, it is shown that TAFII250, the largest subunit of TFIID, contains two tandem bromodomain modules that bind selectively to multiply acetylated histone H4 peptides. The 2.1 angstrom crystal structure of the double bromodomain reveals two side-by-side, four-helix bundles with a highly polarized surface charge distribution. Each bundle contains an Nepsiv -acetyllysine binding pocket at its center, which results in a structure ideally suited for recognition of diacetylated histone H4 tails. Thus, TFIID may be targeted to specific chromatin-bound promoters and may play a role in chromatin recognition.

Howard Hughes Medical Institute and Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, CA 94720-3204, USA.
*   To whom correspondence should be addressed. E-mail: jmlim{at}uclink4.berkeley.edu

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Regulation of Histone Deacetylase 2 by Protein Kinase CK2.
S.-C. Tsai and E. Seto (2002)
J. Biol. Chem. 277, 31826-31833
   Abstract »    Full Text »    PDF »
Reproductive Cycle Regulation of Nuclear Import, Euchromatic Localization, and Association with Components of Pol II Mediator of a Mammalian Double-Bromodomain Protein.
T. E. Crowley, E. M. Kaine, M. Yoshida, A. Nandi, and D. J. Wolgemuth (2002)
Mol. Endocrinol. 16, 1727-1737
   Abstract »    Full Text »    PDF »
Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association.
H. H. Ng, Q. Feng, H. Wang, H. Erdjument-Bromage, P. Tempst, Y. Zhang, and K. Struhl (2002)
Genes & Dev. 16, 1518-1527
   Abstract »    Full Text »    PDF »
Ordered recruitment of histone acetyltransferases and the TRAP/Mediator complex to thyroid hormone-responsive promoters in vivo.
D. Sharma and J. D. Fondell (2002)
PNAS 99, 7934-7939
   Abstract »    Full Text »    PDF »
Synergy among Nuclear Receptor Coactivators: Selective Requirement for Protein Methyltransferase and Acetyltransferase Activities.
Y.-H. Lee, S. S. Koh, X. Zhang, X. Cheng, and M. R. Stallcup (2002)
Mol. Cell. Biol. 22, 3621-3632
   Abstract »    Full Text »    PDF »
Androgen Receptor Acetylation Governs trans Activation and MEKK1-Induced Apoptosis without Affecting In Vitro Sumoylation and trans-Repression Function.
M. Fu, C. Wang, J. Wang, X. Zhang, T. Sakamaki, Y. G. Yeung, C. Chang, T. Hopp, S. A. W. Fuqua, E. Jaffray, et al. (2002)
Mol. Cell. Biol. 22, 3373-3388
   Abstract »    Full Text »    PDF »
Control of IL-2R{alpha} gene expression: structural changes within the proximal enhancer/core promoter during T-cell development.
J.-H. Yeh, S. Spicuglia, S. Kumar, A. Sanchez-Sevilla, P. Ferrier, and J. Imbert (2002)
Nucleic Acids Res. 30, 1944-1951
   Abstract »    Full Text »    PDF »
Isoform-specific interaction of HP1 with human TAFII130.
M. F. Vassallo and N. Tanese (2002)
PNAS 99, 5919-5924
   Abstract »    Full Text »    PDF »
Regulation of transcription by H1 phosphorylation in Tetrahymena is position independent and requires clustered sites.
Y. Dou and M. A. Gorovsky (2002)
PNAS 99, 6142-6146
   Abstract »    Full Text »    PDF »


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