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High Frequency of Hypermutable Pseudomonas aeruginosa in Cystic Fibrosis Lung Infection
Antonio Oliver,
Rafael Cantón,
Pilar Campo,
Fernando Baquero,*
Jesús Blázquez*
The lungs of cystic fibrosis (CF) patients are chronically infected
for years by one or a few lineages of Pseudomonas
aeruginosa.These bacterial populations adapt to the highly
compartmentalizedand anatomically deteriorating lung environment of CF
patients,as well as to the challenges of the immune defenses and
antibiotictherapy. These selective conditions are precisely those that
recenttheoretical studies predict for the evolution of mechanisms thataugment the rate of variation. Determination of spontaneous mutationrates in 128 P. aeruginosa isolates from 30 CF patients
revealedthat 36% of the patients were colonized by a hypermutable
(mutator)strain that persisted for years in most patients. Mutator
strainswere not found in 75 non-CF patients acutely infected with
P.aeruginosa. This investigation also reveals a link
between highmutation rates in vivo and the evolution of antibiotic
resistance.
Department of Microbiology and Unit for Cystic Fibrosis, Hospital
Ramón y Cajal, National Institute of Health (INSALUD), 28034 Madrid, Spain.
*
To whom correspondence should be addressed. E-mail:
fbaquero{at}hrc.insalud.es and
jblazquez{at}hrc.insalud.es
The editors suggest the following Related Resources on Science sites:
In Science Magazine
LETTERS
J. Eugene LeClerc, Thomas A. Cebula;, Jesús Blázquez, Antonio Oliver, and Fernando Baquero (21 July 2000) Science289 (5478), 391c.
[DOI: 10.1126/science.289.5478.391c] |Full Text »
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