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Science 12 May 2000:
Vol. 288. no. 5468, pp. 1026 - 1029
DOI: 10.1126/science.288.5468.1026
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Reports

Separation of Long DNA Molecules in a Microfabricated Entropic Trap Array

J. Han, H. G. Craighead *

A nanofluidic channel device, consisting of many entropic traps, was designed and fabricated for the separation of long DNA molecules. The channel comprises narrow constrictions and wider regions that cause size-dependent trapping of DNA at the onset of a constriction. This process creates electrophoretic mobility differences, thus enabling efficient separation without the use of a gel matrix or pulsed electric fields. Samples of long DNA molecules (5000 to ~160,000 base pairs) were efficiently separated into bands in 15-millimeter-long channels. Multiple-channel devices operating in parallel were demonstrated. The efficiency, compactness, and ease of fabrication of the device suggest the possibility of more practical integrated DNA analysis systems.

School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
*   To whom correspondence should be addressed. E-mail: hgc1{at}cornell.edu

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