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Science 28 April 2000:
Vol. 288. no. 5466, pp. 665 - 669
DOI: 10.1126/science.288.5466.665
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Reports

Extension of Cell Life-Span and Telomere Length in Animals Cloned from Senescent Somatic Cells

Robert P. Lanza, 1* Jose B. Cibelli, 1 Catherine Blackwell, 1 Vincent J. Cristofalo, 2 Mary Kay Francis, 2 Gabriela M. Baerlocher, 3 Jennifer Mak, 3 Michael Schertzer, 3 Elizabeth A. Chavez, 3 Nancy Sawyer, 1 Peter M. Lansdorp, 34 Michael D. West 1

The potential of cloning depends in part on whether the procedure can reverse cellular aging and restore somatic cells to a phenotypically youthful state. Here, we report the birth of six healthy cloned calves derived from populations of senescent donor somatic cells. Nuclear transfer extended the replicative life-span of senescent cells (zero to four population doublings remaining) to greater than 90 population doublings. Early population doubling level complementary DNA-1 (EPC-1, an age-dependent gene) expression in cells from the cloned animals was 3.5- to 5-fold higher than that in cells from age-matched (5 to 10 months old) controls. Southern blot and flow cytometric analyses indicated that the telomeres were also extended beyond those of newborn (<2 weeks old) and age-matched control animals. The ability to regenerate animals and cells may have important implications for medicine and the study of mammalian aging.

1 Advanced Cell Technology, One Innovation Drive, Worcester, MA 01605, USA.
2 Lankenau Institute for Medical Research, Wynnewood, PA 19096, and the Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19104, USA.
3 Terry Fox Laboratory, British Columbia Cancer Research Center, 601 West 10 Avenue, Vancouver, BC, V5Z 1L3 Canada.
4 Department of Medicine, University of British Columbia, Vancouver, BC, V6T 2B5 Canada.
*   To whom correspondence should be addressed. E-mail: rlanza{at}advancedcell.com

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