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Science 21 April 2000:
Vol. 288. no. 5465, pp. 517 - 522
DOI: 10.1126/science.288.5465.517
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Reports

Homologs of Small Nucleolar RNAs in Archaea

Arina D. Omer, 1 Todd M. Lowe, 2* Anthony G. Russell, 1 Holger Ebhardt, 1 Sean R. Eddy, 2 Patrick P. Dennis 1dagger

In eukaryotes, dozens of posttranscriptional modifications are directed to specific nucleotides in ribosomal RNAs (rRNAs) by small nucleolar RNAs (snoRNAs). We identified homologs of snoRNA genes in both branches of the Archaea. Eighteen small sno-like RNAs (sRNAs) were cloned from the archaeon Sulfolobus acidocaldarius by coimmunoprecipitation with archaeal fibrillarin and NOP56, the homologs of eukaryotic snoRNA-associated proteins. We trained a probabilistic model on these sRNAs to search for more sRNAs in archaeal genomic sequences. Over 200 additional sRNAs were identified in seven archaeal genomes representing both the Crenarchaeota and the Euryarchaeota. snoRNA-based rRNA processing was therefore probably present in the last common ancestor of Archaea and Eukarya, predating the evolution of a morphologically distinct nucleolus.

1 Department of Biochemistry and Molecular Biology, University of British Columbia, 2146 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada.
2 Department of Genetics, Washington University School of Medicine, 4566 Scott Avenue, St. Louis, MO 63110, USA.
*   Present address: Department of Genetics, M322, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305-5120, USA.

dagger    To whom correspondence should be addressed. E-mail: patrick.p.dennis{at}ubc.ca

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The complete genome of the crenarchaeon Sulfolobus solfataricus P2.
Q. She, R. K. Singh, F. Confalonieri, Y. Zivanovic, G. Allard, M. J. Awayez, C. C.-Y. Chan-Weiher, I. G. Clausen, B. A. Curtis, A. De Moors, et al. (2001)
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From the Cover: Genome sequence of Halobacterium species NRC-1.
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Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.
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