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Science 14 April 2000:
Vol. 288. no. 5464, pp. 344 - 349
DOI: 10.1126/science.288.5464.344
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Reports

Regulation of Area Identity in the Mammalian Neocortex by Emx2 and Pax6

Kathie M. Bishop, 1 Guy Goudreau, 2 Dennis D. M. O'Leary 1*

The contribution of extrinsic and genetic mechanisms in determining areas of the mammalian neocortex has been a contested issue. This study analyzes the roles of the regulatory genes Emx2 and Pax6, which are expressed in opposing gradients in the neocortical ventricular zone, in specifying areas. Changes in the patterning of molecular markers and area-specific connections between the cortex and thalamus suggest that arealization of the neocortex is disproportionately altered in Emx2 and Pax6 mutant mice in opposing manners predicted from their countergradients of expression: rostral areas expand and caudal areas contract in Emx2 mutants, whereas the opposite effect is seen in Pax6 mutants. These findings suggest that Emx2 and Pax6 cooperate to regulate arealization of the neocortex and to confer area identity to cortical cells.

1 Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
2 Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Cell Biology, Am Fassberg 11, 37077 Göttingen, Germany.
*   To whom correspondence should be addressed. E-mail: doleary{at}salk.edu

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