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Science 14 April 2000:
Vol. 288. no. 5464, pp. 316 - 318
DOI: 10.1126/science.288.5464.316
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Reports

Translating Biomolecular Recognition into Nanomechanics

J. Fritz, 12 M. K. Baller, 12 H. P. Lang, 12 H. Rothuizen, 1 P. Vettiger, 1 E. Meyer, 2 H. -J. Güntherodt, 2 Ch. Gerber, 1* J. K. Gimzewski 1

We report the specific transduction, via surface stress changes, of DNA hybridization and receptor-ligand binding into a direct nanomechanical response of microfabricated cantilevers. Cantilevers in an array were functionalized with a selection of biomolecules. The differential deflection of the cantilevers was found to provide a true molecular recognition signal despite large nonspecific responses of individual cantilevers. Hybridization of complementary oligonucleotides shows that a single base mismatch between two 12-mer oligonucleotides is clearly detectable. Similar experiments on protein A-immunoglobulin interactions demonstrate the wide-ranging applicability of nanomechanical transduction to detect biomolecular recognition.

1 IBM Research, Zurich Research Laboratory, Säumerstrasse 4, CH-8803 Rüschlikon, Switzerland.
2 Physics Institute, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland.
*   To whom correspondence should be addressed. E-mail: ge{at}zurich.ibm.com

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