Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 7 April 2000:
Vol. 288. no. 5463, pp. 146 - 149
DOI: 10.1126/science.288.5463.146
Prev | Table of Contents | Next

Reports

Specification of Drosophila Hematopoietic Lineage by Conserved Transcription Factors

Tim Lebestky, 1* Ting Chang, 2* Volker Hartenstein, 12 Utpal Banerjee 123dagger

Two major classes of cells observed within the Drosophila hematopoietic repertoire are plasmatocytes/macrophages and crystal cells. The transcription factor Lz (Lozenge), which resembles human AML1 (acute myeloid leukemia- 1) protein, is necessary for the development of crystal cells during embryonic and larval hematopoiesis. Another transcription factor, Gcm (glial cells missing), has previously been shown to be required for plasmatocyte development. Misexpression of Gcm causes crystal cells to be transformed into plasmatocytes. The Drosophila GATA protein Srp (Serpent) is required for both Lz and Gcm expression and is necessary for the development of both classes of hemocytes, whereas Lz and Gcm are required in a lineage-specific manner. Given the similarities of Srp and Lz to mammalian GATA and AML1 proteins, observations in Drosophila are likely to have broad implications for understanding mammalian hematopoiesis and leukemias.

1 Molecular Biology Institute,
2 Department of Molecular, Cell, and Developmental Biology,
3 Departments of Biological Chemistry and Human Genetics, University of California, Los Angeles, CA 90095, USA.
*   These authors contributed equally to this report.

dagger    To whom correspondence should be addressed. E-mail: banerjee{at}mbi.ucla.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Preclinical testing on insects predicts human haematotoxic potentials.
J. Berger (2009)
Lab Anim 43, 328-332
   Abstract »    Full Text »    PDF »
A Drosophila model identifies calpains as modulators of the human leukemogenic fusion protein AML1-ETO.
D. Osman, V. Gobert, F. Ponthan, O. Heidenreich, M. Haenlin, and L. Waltzer (2009)
PNAS 106, 12043-12048
   Abstract »    Full Text »    PDF »
Elimination of plasmatocytes by targeted apoptosis reveals their role in multiple aspects of the Drosophila immune response.
B. Charroux and J. Royet (2009)
PNAS 106, 9797-9802
   Abstract »    Full Text »    PDF »
Requirement of Split ends for Epigenetic Regulation of Notch Signal-Dependent Genes during Infection-Induced Hemocyte Differentiation.
L. H. Jin, J. K. Choi, B. Kim, H. S. Cho, J. Kim, J. Kim-Ha, and Y.-J. Kim (2009)
Mol. Cell. Biol. 29, 1515-1525
   Abstract »    Full Text »    PDF »
Identification of the Drosophila core 1 {beta}1,3-galactosyltransferase gene that synthesizes T antigen in the embryonic central nervous system and hemocytes.
H. Yoshida, T. J Fuwa, M. Arima, H. Hamamoto, N. Sasaki, T. Ichimiya, K.-i. Osawa, R. Ueda, and S. Nishihara (2008)
Glycobiology 18, 1094-1104
   Abstract »    Full Text »    PDF »
An innate immune response of blood cells to tumors and tissue damage in Drosophila.
J. C. Pastor-Pareja, M. Wu, and T. Xu (2008)
Dis. Model. Mech. 1, 144-154
   Abstract »    Full Text »    PDF »
Two Subunits Specific to the PBAP Chromatin Remodeling Complex Have Distinct and Redundant Functions during Drosophila Development.
I. Carrera, J. Zavadil, and J. E. Treisman (2008)
Mol. Cell. Biol. 28, 5238-5250
   Abstract »    Full Text »    PDF »
A Misexpression Screen to Identify Regulators of Drosophila Larval Hemocyte Development.
M. Stofanko, S. Y. Kwon, and P. Badenhorst (2008)
Genetics 180, 253-267
   Abstract »    Full Text »    PDF »
Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification.
Y. Guo, I. Maillard, S. Chakraborti, E. V. Rothenberg, and N. A. Speck (2008)
Blood 112, 480-492
   Abstract »    Full Text »    PDF »
Haemocyte-derived SPARC is required for collagen-IV-dependent stability of basal laminae in Drosophila embryos.
N. Martinek, J. Shahab, M. Saathoff, and M. Ringuette (2008)
J. Cell Sci. 121, 1671-1680
   Abstract »    Full Text »    PDF »
Identification of Integrin-{alpha}4, Rb1, and Syncytin A as Murine Placental Target Genes of the Transcription Factor GCMa/Gcm1.
S. W. Schubert, N. Lamoureux, K. Kilian, L. Klein-Hitpass, and S. Hashemolhosseini (2008)
J. Biol. Chem. 283, 5460-5465
   Abstract »    Full Text »    PDF »
The hematopoietic stem cell and its niche: a comparative view.
J. A. Martinez-Agosto, H. K.A. Mikkola, V. Hartenstein, and U. Banerjee (2007)
Genes & Dev. 21, 3044-3060
   Abstract »    Full Text »    PDF »
Senseless functions as a molecular switch for color photoreceptor differentiation in Drosophila.
B. Xie, M. Charlton-Perkins, E. McDonald, B. Gebelein, and T. Cook (2007)
Development 134, 4243-4253
   Abstract »    Full Text »    PDF »
Drosophila Hemopoiesis and Cellular Immunity.
M. J. Williams (2007)
J. Immunol. 178, 4711-4716
   Abstract »    Full Text »    PDF »
GATA-4 Incompletely Substitutes for GATA-1 in Promoting Both Primitive and Definitive Erythropoiesis in Vivo.
S. Hosoya-Ohmura, N. Mochizuki, M. Suzuki, O. Ohneda, K. Ohneda, and M. Yamamoto (2006)
J. Biol. Chem. 281, 32820-32830
   Abstract »    Full Text »    PDF »
Melanotic Mutants in Drosophila: Pathways and Phenotypes.
S. Minakhina and R. Steward (2006)
Genetics 174, 253-263
   Abstract »    Full Text »    PDF »
Hand, an evolutionarily conserved bHLH transcription factor required for Drosophila cardiogenesis and hematopoiesis.
Z. Han, P. Yi, X. Li, and E. N. Olson (2006)
Development 133, 1175-1182
   Abstract »    Full Text »    PDF »
Resolving embryonic blood cell fate choice in Drosophila: interplay of GCM and RUNX factors.
L. Bataille, B. Auge, G. Ferjoux, M. Haenlin, and L. Waltzer (2005)
Development 132, 4635-4644
   Abstract »    Full Text »    PDF »
Hand is a direct target of Tinman and GATA factors during Drosophila cardiogenesis and hematopoiesis.
Z. Han and E. N. Olson (2005)
Development 132, 3525-3536
   Abstract »    Full Text »    PDF »
The Drosophila lymph gland as a developmental model of hematopoiesis.
S.-H. Jung, C. J. Evans, C. Uemura, and U. Banerjee (2005)
Development 132, 2521-2533
   Abstract »    Full Text »    PDF »
The t(8;21) translocation converts AML1 into a constitutive transcriptional repressor.
J. Wildonger and R. S. Mann (2005)
Development 132, 2263-2272
   Abstract »    Full Text »    PDF »
Functional Evolution of the Vertebrate Myb Gene Family: B-Myb, but Neither A-Myb nor c-Myb, Complements Drosophila Myb in Hemocytes.
C. J. Davidson, R. Tirouvanziam, L. A. Herzenberg, and J. S. Lipsick (2005)
Genetics 169, 215-229
   Abstract »    Full Text »    PDF »
Function of Rho GTPases in embryonic blood cell migration in Drosophila.
M. Paladi and U. Tepass (2004)
J. Cell Sci. 117, 6313-6326
   Abstract »    Full Text »    PDF »
Interaction, Cooperative Promoter Modulation, and Renal Colocalization of GCMa and Pitx2.
S. W. Schubert, E. Kardash, M. A. Khan, T. Cheusova, K. Kilian, M. Wegner, and S. Hashemolhosseini (2004)
J. Biol. Chem. 279, 50358-50365
   Abstract »    Full Text »    PDF »
A directed screen for genes involved in Drosophila blood cell activation.
C.-J. Zettervall, I. Anderl, M. J. Williams, R. Palmer, E. Kurucz, I. Ando, and D. Hultmark (2004)
PNAS 101, 14192-14197
   Abstract »    Full Text »    PDF »
Identification and Characterization of Genes Involved in Embryonic Crystal Cell Formation During Drosophila Hematopoiesis.
A. B. Milchanowski, A. L. Henkenius, M. Narayanan, V. Hartenstein, and U. Banerjee (2004)
Genetics 168, 325-339
   Abstract »    Full Text »    PDF »
Positioning sensory terminals in the olfactory lobe of Drosophila by Robo signaling.
D. Jhaveri, S. Saharan, A. Sen, and V. Rodrigues (2004)
Development 131, 1903-1912
   Abstract »    Full Text »    PDF »
Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes.
R. Tirouvanziam, C. J. Davidson, J. S. Lipsick, and L. A. Herzenberg (2004)
PNAS 101, 2912-2917
   Abstract »    Full Text »    PDF »
Functional overlap of GATA-1 and GATA-2 in primitive hematopoietic development.
Y. Fujiwara, A. N. Chang, A. M. Williams, and S. H. Orkin (2004)
Blood 103, 583-585
   Abstract »    Full Text »    PDF »
The two origins of hemocytes in Drosophila.
A. Holz, B. Bossinger, T. Strasser, W. Janning, and R. Klapper (2003)
Development 130, 4955-4962
   Abstract »    Full Text »    PDF »
Patterning of the cardiac outflow region in Drosophila.
M. Zikova, J.-P. Da Ponte, B. Dastugue, and K. Jagla (2003)
PNAS 100, 12189-12194
   Abstract »    Full Text »    PDF »
Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening.
C. Calestani, J. P. Rast, and E. H. Davidson (2003)
Development 130, 4587-4596
   Abstract »    Full Text »    PDF »
Combinatorial interactions of Serpent, Lozenge, and U-shaped regulate crystal cell lineage commitment during Drosophila hematopoiesis.
N. Fossett, K. Hyman, K. Gajewski, S. H. Orkin, and R. A. Schulz (2003)
PNAS 100, 11451-11456
   Abstract »    Full Text »    PDF »
Macrophage-mediated corpse engulfment is required for normal Drosophila CNS morphogenesis.
H. C. Sears, C. J. Kennedy, and P. A. Garrity (2003)
Development 130, 3557-3565
   Abstract »    Full Text »    PDF »
Drosophila homeodomain protein REPO controls glial differentiation by cooperating with ETS and BTB transcription factors.
Y. Yuasa, M. Okabe, S. Yoshikawa, K. Tabuchi, W.-C. Xiong, Y. Hiromi, and H. Okano (2003)
Development 130, 2419-2428
   Abstract »    Full Text »    PDF »
RUNX1 and GATA-1 coexpression and cooperation in megakaryocytic differentiation.
K. E. Elagib, F. K. Racke, M. Mogass, R. Khetawat, L. L. Delehanty, and A. N. Goldfarb (2003)
Blood 101, 4333-4341
   Abstract »    Full Text »    PDF »
Gliogenesis in Drosophila: genome-wide analysis of downstream genes of glial cells missing in the embryonic nervous system.
B. Egger, R. Leemans, T. Loop, L. Kammermeier, Y. Fan, T. Radimerski, M. C. Strahm, U. Certa, and H. Reichert (2003)
Development 129, 3295-3309
   Abstract »    Full Text »    PDF »
Runx1 is required for zebrafish blood and vessel development and expression of a human RUNX1-CBF2T1 transgene advances a model for studies of leukemogenesis.
M. L. Kalev-Zylinska, J. A. Horsfield, M. V. C. Flores, J. H. Postlethwait, M. R. Vitas, A. M. Baas, P. S. Crosier, and K. E. Crosier (2003)
Development 129, 2015-2030
   Abstract »    Full Text »    PDF »
A Serrate-expressing signaling center controls Drosophila hematopoiesis.
T. Lebestky, S.-H. Jung, and U. Banerjee (2003)
Genes & Dev. 17, 348-353
   Abstract »    Full Text »    PDF »
Regulation of Larval Hematopoiesis in Drosophila melanogaster: A Role for the multi sex combs Gene.
N. Remillieux-Leschelle, P. Santamaria, and N. B. Randsholt (2002)
Genetics 162, 1259-1274
   Abstract »    Full Text »    PDF »
A human Mix-like homeobox gene MIXL shows functional similarity to Xenopus Mix.1.
W. Guo, A. Pui-yee Chan, H. Liang, E. D. Wieder, J. J. Molldrem, L. D. Etkin, and L. Nagarajan (2002)
Blood 100, 89-95
   Abstract »    Full Text »    PDF »
Drosophila Immunity: Genes on the Third Chromosome Required for the Response to Bacterial Infection.
L. P. Wu, K.-M. Choe, Y. Lu, and K. V. Anderson (2001)
Genetics 159, 189-199
   Abstract »    Full Text »    PDF »
Redundant function of Runt Domain binding partners, Big brother and Brother, during Drosophila development.
J. S. Kaminker, R. Singh, T. Lebestky, H. Yan, and U. Banerjee (2001)
Development 128, 2639-2648
   Abstract »    Full Text »    PDF »
A UAS site substitution approach to the in vivo dissection of promoters: interplay between the GATAb activator and the AEF-1 repressor at a Drosophila ecdysone response unit.
V. Brodu, B. Mugat, P. Fichelson, J.-A. Lepesant, and C. Antoniewski (2001)
Development 128, 2593-2602
   Abstract »    Full Text »    PDF »
The Friend of GATA proteins U-shaped, FOG-1, and FOG-2 function as negative regulators of blood, heart, and eye development in Drosophila.
N. Fossett, S. G. Tevosian, K. Gajewski, Q. Zhang, S. H. Orkin, and R. A. Schulz (2001)
PNAS
   Abstract »    Full Text »    PDF »
The Friend of GATA proteins U-shaped, FOG-1, and FOG-2 function as negative regulators of blood, heart, and eye development in Drosophila.
N. Fossett, S. G. Tevosian, K. Gajewski, Q. Zhang, S. H. Orkin, and R. A. Schulz (2001)
PNAS 98, 7342-7347
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)