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Science 24 March 2000:
Vol. 287. no. 5461, pp. 2260 - 2262
DOI: 10.1126/science.287.5461.2260
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Reports

Positioning of the Mitotic Spindle by a Cortical-Microtubule Capture Mechanism

Laifong Lee, 12 Jennifer S. Tirnauer, 1 Junjun Li, 1 Scott C. Schuyler, 1 Jenny Y. Liu, 1 David Pellman 1*

Correct positioning of the mitotic spindle is critical for cell division and development. Spindle positioning involves a search-and-capture mechanism whereby dynamic microtubules find and then interact with specific sites on the submembrane cortex. Genetic, biochemical, and imaging experiments suggest a mechanism for cortical-microtubule capture. Bim1p, located at microtubule distal ends, bound Kar9p, a protein associated with the daughter cell cortex. Bim1p is the yeast ortholog of human EB1, a binding partner for the adenomatous polyposis coli tumor suppressor. EB1 family proteins may have a general role in linking the microtubule cytoskeleton to cortical polarity determinants.

1 Departments of Pediatric Oncology, The Dana-Farber Cancer Institute, and Pediatric Hematology, The Children's Hospital, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
2 Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
*   To whom correspondence should be addressed. E-mail: david_pellman{at}dfci.harvard.edu

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