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Science 3 March 2000:
Vol. 287. no. 5458, pp. 1641 - 1644
DOI: 10.1126/science.287.5458.1641
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Reports

A Potassium Channel Protein Encoded by Chlorella Virus PBCV-1

B. Plugge, 1* S. Gazzarrini, 2* M. Nelson, 3* R. Cerana, 4 J. L. Van Etten, 5 C. Derst, 6 D. DiFrancesco, 2 A. Moroni, 2 G. Thiel 1dagger

The large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus life cycle.

1 Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen, 37073 Göttingen, Germany.
2 Università degli Studi di Milano, 20133 Milan, Italy.
3 Megabase Research Products, 4711 Huntington Avenue, Lincoln, NE 68604, USA.
4 DISAT, Università di Milano Bicocca, 20126 Milan, Italy.
5 Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583-0722, USA.
6 Institut für Physiologie, Philipps-Universität Marburg, 35033 Marburg, Germany.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: gthiel{at}gwdvms.gwdg.de

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