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Science 25 February 2000:
Vol. 287. no. 5457, pp. 1479 - 1482
DOI: 10.1126/science.287.5457.1479
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Reports

Effects of Environment on Compensatory Mutations to Ameliorate Costs of Antibiotic Resistance

J. Björkman, 12* I. Nagaev, 2* O. G. Berg, 3 D. Hughes, 2 D. I. Andersson 1dagger

Most types of antibiotic resistance impose a biological cost on bacterial fitness. These costs can be compensated, usually without loss of resistance, by second-site mutations during the evolution of the resistant bacteria in an experimental host or in a laboratory medium. Different fitness-compensating mutations were selected depending on whether the bacteria evolved through serial passage in mice or in a laboratory medium. This difference in mutation spectra was caused by either a growth condition-specific formation or selection of the compensated mutants. These results suggest that bacterial evolution to reduce the costs of antibiotic resistance can take different trajectories within and outside a host.

1 Department of Bacteriology, Swedish Institute for Infectious Disease Control, S-171 82 Solna, Sweden.
2 Department of Cell and Molecular Biology, Uppsala University, Biomedical Centre (BMC), Box 596, S-751 24 Uppsala, Sweden.
3 Department of Molecular Evolution, Uppsala University, BMC, Box 582, S-751 23 Uppsala, Sweden.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: Dan.Andersson{at}smi.ki.se

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