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Science 21 January 2000:
Vol. 287. no. 5452, pp. 501 - 504
DOI: 10.1126/science.287.5452.501
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Reports

Rad6-Dependent Ubiquitination of Histone H2B in Yeast

Kenneth Robzyk, Judith Recht, * Mary Ann Osley dagger

Although ubiquitinated histones are present in substantial levels in vertebrate cells, the roles they play in specific biological processes and the cellular factors that regulate this modification are not well characterized. Ubiquitinated H2B (uH2B) has been identified in the yeast Saccharomyces cerevisiae, and mutation of the conserved ubiquitination site is shown to confer defects in mitotic cell growth and meiosis. uH2B was not detected in rad6 mutants, which are defective for the ubiquitin-conjugating enzyme Ubc2, thus identifying Rad6 as the major cellular activity that ubiquitinates H2B in yeast.

Program in Molecular Biology, Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.
*   Present address: Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA.

dagger    To whom correspondence should be addressed. E-mail: m-osley{at}ski.mskcc.org

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The Saccharomyces cerevisiae RAD6 Group Is Composed of an Error-Prone and Two Error-Free Postreplication Repair Pathways.
W. Xiao, B. L. Chow, S. Broomfield, and M. Hanna (2000)
Genetics 155, 1633-1641
   Abstract »    Full Text »
Evidence for an Interaction between Ubiquitin-Conjugating Enzymes and the 26S Proteasome.
P. Tongaonkar, L. Chen, D. Lambertson, B. Ko, and K. Madura (2000)
Mol. Cell. Biol. 20, 4691-4698
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Human mdm2 Mediates Multiple Mono-ubiquitination of p53 by a Mechanism Requiring Enzyme Isomerization.
Z. Lai, K. V. Ferry, M. A. Diamond, K. E. Wee, Y. B. Kim, J. Ma, T. Yang, P. A. Benfield, R. A. Copeland, and K. R. Auger (2001)
J. Biol. Chem. 276, 31357-31367
   Abstract »    Full Text »    PDF »
Distinct Functional Surface Regions on Ubiquitin.
K. E. Sloper-Mould, J. C. Jemc, C. M. Pickart, and L. Hicke (2001)
J. Biol. Chem. 276, 30483-30489
   Abstract »    Full Text »    PDF »
Multiple Roles for Rsp5p-dependent Ubiquitination at the Internalization Step of Endocytosis.
R. Dunn and L. Hicke (2001)
J. Biol. Chem. 276, 25974-25981
   Abstract »    Full Text »    PDF »
The Ubiquitin-like Protein FAT10 Forms Covalent Conjugates and Induces Apoptosis.
S. Raasi, G. Schmidtke, and M. Groettrup (2001)
J. Biol. Chem. 276, 35334-35343
   Abstract »    Full Text »    PDF »


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