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Science 21 January 2000:
Vol. 287. no. 5452, pp. 495 - 498
DOI: 10.1126/science.287.5452.495
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Reports

A Role for Histone Acetylation in the Developmental Regulation of V(D)J Recombination

Michelle Taylor McMurry, Michael S. Krangel *

V(D)J recombination is developmentally regulated in vivo by enhancer-dependent changes in the accessibility of chromosomal recombination signal sequences to the recombinase, but the molecular nature of these changes is unknown. Here histone H3 acetylation was measured along versions of a transgenic V(D)J recombination reporter and the endogenous T cell receptor alpha /delta locus. Enhancer activity was shown to impart long-range, developmentally regulated changes in H3 acetylation, and H3 acetylation status was tightly linked to V(D)J recombination. H3 hyperacetylation is proposed as a molecular mechanism coupling enhancer activity to accessibility for V(D)J recombination.

Department of Immunology, Post Office Box 3010, Duke University Medical Center, Durham NC 27710, USA.
*   To whom correspondence should be addressed. E-mail: krang001{at}mc.duke.edu

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Control of chromatin accessibility for V(D)J recombination by interleukin-7.
J. Huang and K. Muegge (2001)
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D. L. Chalker and M.-C. Yao (2001)
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J. Exp. Med. 193, 873-880
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V(D)J Recombination Becomes Accessible.
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J. Exp. Med. 193, F27-F30
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J. Immunol. 166, 4552-4559
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Induction of a Diverse T Cell Receptor {{gamma}}/{{delta}} Repertoire by the Helix-Loop-Helix Proteins E2A and HEB in Nonlymphoid Cells.
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Insertion of Phosphoglycerine Kinase (PGK)-Neo 5' of J{{lambda}}1 Dramatically Enhances VJ{{lambda}}1 Rearrangement.
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J. Exp. Med. 193, 699-712
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Matrix Attachment Region-Dependent Function of the Immunoglobulin {micro} Enhancer Involves Histone Acetylation at a Distance without Changes in Enhancer Occupancy.
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   Abstract »    Full Text »
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   Abstract »    Full Text »
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J. Exp. Med. 192, 625-636
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Acetylation of Histones and Transcription-Related Factors.
D. E. Sterner and S. L. Berger (2000)
Microbiol. Mol. Biol. Rev. 64, 435-459
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M. A. Nikiforov, M. A. Gorovsky, and C. D. Allis (2000)
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J. Landry, A. Sutton, S. T. Tafrov, R. C. Heller, J. Stebbins, L. Pillus, and R. Sternglanz (2000)
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The murine beta -globin locus control region regulates the rate of transcription but not the hyperacetylation of histones at the active genes.
D. Schubeler, M. Groudine, and M. A. Bender (2001)
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