Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 14 January 2000:
Vol. 287. no. 5451, pp. 310 - 314
DOI: 10.1126/science.287.5451.310
Prev | Table of Contents | Next

Reports

Crystal Structure of a gamma &dgr; T Cell Receptor Ligand T22: A Truncated MHC-Like Fold

Christer Wingren, 1* Michael P. Crowley, 2 † Massimo Degano, 1ddagger Yueh-hsiu Chien, 2 Ian A. Wilson 1§

Murine T10 and T22 are highly related nonclassical major histocompatibility complex (MHC) class Ib proteins that bind to certain gamma delta T cell receptors (TCRs) in the absence of other components. The crystal structure of T22b at 3.1 angstroms reveals similarities to MHC class I molecules, but one side of the normal peptide-binding groove is severely truncated, which allows direct access to the beta -sheet floor. Potential gamma delta TCR-binding sites can be inferred from functional mapping of T10 and T22 point mutants and allelic variants. Thus, T22 represents an unusual variant of the MHC-like fold and indicates that gamma delta and alpha beta TCRs interact differently with their respective MHC ligands.

1 Department of Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
2 Program of Immunology and the Department of Microbiology and Immunology, Stanford University, School of Medicine, Stanford, CA 94305, USA.
*   Present address: Department of Immunotechnology, Lund University, Post Office Box 7031, SE-22007 Lund, Sweden.

ddagger    Present address: Structural Biology Laboratory, Sincrotrone Trieste in Area Science Park, S.S. 14 Km 163.5, 34012 Basovizza (TS), Italy.

§   To whom correspondence should be addressed. E-mail: wilson{at}scripps.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Structural elucidation of the m157 mouse cytomegalovirus ligand for Ly49 natural killer cell receptors.
E. J. Adams, Z. S. Juo, R. T. Venook, M. J. Boulanger, H. Arase, L. L. Lanier, and K. C. Garcia (2007)
PNAS 104, 10128-10133
   Abstract »    Full Text »    PDF »
MHC Class I-Like MILL Molecules Are beta2-Microglobulin-Associated, GPI-Anchored Glycoproteins That Do Not Require TAP for Cell Surface Expression..
M. Kajikawa, T. Baba, U. Tomaru, Y. Watanabe, S. Koganei, S. Tsuji-Kawahara, N. Matsumoto, K. Yamamoto, M. Miyazawa, K. Maenaka, et al. (2006)
J. Immunol. 177, 3108-3115
   Abstract »    Full Text »    PDF »
Evidence for MR1 Antigen Presentation to Mucosal-associated Invariant T Cells.
S. Huang, S. Gilfillan, M. Cella, M. J. Miley, O. Lantz, L. Lybarger, D. H. Fremont, and T. H. Hansen (2005)
J. Biol. Chem. 280, 21183-21193
   Abstract »    Full Text »    PDF »
Structure of a {gamma}{delta} T Cell Receptor in Complex with the Nonclassical MHC T22.
E. J. Adams, Y.-H. Chien, and K. C. Garcia (2005)
Science 308, 227-231
   Abstract »    Full Text »    PDF »
Infection-Induced Expansion of a MHC Class Ib-Dependent Intestinal Intraepithelial {gamma}{delta} T Cell Subset.
A. Davies, S. Lopez-Briones, H. Ong, C. O'Neil-Marshall, F. A. Lemonnier, K. Nagaraju, E. S. Metcalf, and M. J. Soloski (2004)
J. Immunol. 172, 6828-6837
   Abstract »    Full Text »    PDF »
Biochemical Features of the MHC-Related Protein 1 Consistent with an Immunological Function.
M. J. Miley, S. M. Truscott, Y. Y. L. Yu, S. Gilfillan, D. H. Fremont, T. H. Hansen, and L. Lybarger (2003)
J. Immunol. 170, 6090-6098
   Abstract »    Full Text »    PDF »
Memory Phenotype of CD8+ T Cells in MHC Class Ia-Deficient Mice.
Z. Kurepa, J. Su, and J. Forman (2003)
J. Immunol. 170, 5414-5420
   Abstract »    Full Text »    PDF »
Peptide-Independent Folding and CD8{alpha}{alpha} Binding by the Nonclassical Class I Molecule, Thymic Leukemia Antigen.
D. A. Weber, A. Attinger, C. C. Kemball, J. L. Wigal, J. Pohl, Y. Xiong, E. L. Reinherz, H. Cheroutre, M. Kronenberg, and P. E. Jensen (2002)
J. Immunol. 169, 5708-5714
   Abstract »    Full Text »    PDF »
Structural Studies of Allelic Diversity of the MHC Class I Homolog MIC-B, a Stress-Inducible Ligand for the Activating Immunoreceptor NKG2D.
M. A. Holmes, P. Li, E. W. Petersdorf, and R. K. Strong (2002)
J. Immunol. 169, 1395-1400
   Abstract »    Full Text »    PDF »
MHC and MHC-related proteins as pleiotropic signal molecules.
D. M. OJCIUS, C. DELARBRE, P. KOURILSKY, and G. GACHELIN (2002)
FASEB J 16, 202-206
   Abstract »    Full Text »    PDF »
Essential Contribution of Germline-Encoded Lysine Residues in J{gamma}1.2 Segment to the Recognition of Nonpeptide Antigens by Human {gamma}{delta} T Cells.
F. Miyagawa, Y. Tanaka, S. Yamashita, B. Mikami, K. Danno, M. Uehara, and N. Minato (2001)
J. Immunol. 167, 6773-6779
   Abstract »    Full Text »    PDF »
A Population of Murine T Cells That Recognize an Inducible MHC Class Ib Molecule.
M. P. Crowley, A. M. Fahrer, N. Baumgarth, J. Hampl, I. Gutgemann, L. Teyton, and Y. Chien (2000)
Science 287, 314-316
   Abstract »    Full Text »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)