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Science 14 January 2000:
Vol. 287. no. 5451, pp. 300 - 303
DOI: 10.1126/science.287.5451.300
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Reports

Regulation of Abscisic Acid-Induced Stomatal Closure and Anion Channels by Guard Cell AAPK Kinase

Jiaxu Li, Xi-Qing Wang, Mark B. Watson, Sarah M. Assmann *

Abscisic acid (ABA) stimulates stomatal closure and thus supports water conservation by plants during drought. Mass spectrometry-generated peptide sequence information was used to clone a Vicia faba complementary DNA, AAPK, encoding a guard cell-specific ABA-activated serine-threonine protein kinase (AAPK). Expression in transformed guard cells of AAPK altered by one amino acid (lysine 43 to alanine 43) renders stomata insensitive to ABA-induced closure by eliminating ABA activation of plasma membrane anion channels. This information should allow cell-specific, targeted biotechnological manipulation of crop water status.

Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA.
*   To whom correspondence should be addressed. E-mail: sma3{at}psu.edu

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Plant Physiology 128, 714-725
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S. H. Yang, T. Berberich, H. Sano, and T. Kusano (2001)
Plant Physiology 127, 23-32
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X. Zhang, L. Zhang, F. Dong, J. Gao, D. W. Galbraith, and C.-P. Song (2001)
Plant Physiology 126, 1438-1448
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G Protein Regulation of Ion Channels and Abscisic Acid Signaling in Arabidopsis Guard Cells.
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Science 292, 2070-2072
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J.-U. Hwang and Y. Lee (2001)
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Plant Physiology 125, 139-141
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Plant Physiology 123, 807-810
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J. Biol. Chem. 276, 9855-9860
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KAT1 is not essential for stomatal opening.
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PNAS 98, 2917-2921
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PNAS 98, 14138-14143
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