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Science 7 January 2000:
Vol. 287. no. 5450, pp. 138 - 142
DOI: 10.1126/science.287.5450.138
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Reports

A Mammalian H+ Channel Generated Through Alternative Splicing of the NADPH Oxidase Homolog NOH-1

Botond Bánfi, 5 Andrés Maturana, 23 Stefano Jaconi, Serge Arnaudeau, 2 Terese Laforge, Bhanu Sinha, 4 Erzsébet Ligeti, 5 Nicolas Demaurex, 2* Karl-Heinz Krause

Voltage-gated proton (H+) channels are found in many human and animal tissues and play an important role in cellular defense against acidic stress. However, a molecular identification of these unique ion conductances has so far not been achieved. A 191-amino acid protein is described that, upon heterologous expression, has properties indistinguishable from those of native H+ channels. This protein is generated through alternative splicing of messenger RNA derived from the gene NOH-1 (NADPH oxidase homolog 1, where NADPH is the reduced form of nicotinamide adenine dinucleotide phosphate).

1Biology of Aging Laboratory, Department of Geriatrics,
2 Department of Physiology,
3 Fondation pour Recherches Médicales,
4 Division of Infectious Diseases, Geneva University Hospitals, Geneva Medical School, CH-1211 Geneva 4, Switzerland.
5 Department of Physiology, Semmelweis Medical University, H-1444 Budapest 8, Hungary.
*   To whom correspondence should be addressed. E-mail: Nicolas.Demaurex{at}medecine.unige.ch

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J. Biol. Chem. 276, 30277-30284
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