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Science 17 December 1999:
Vol. 286. no. 5448, pp. 2342 - 2344
DOI: 10.1126/science.286.5448.2342
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Reports

Posttranscriptional Gene Silencing in Neurospora by a RecQ DNA Helicase

Carlo Cogoni, * Giuseppe Macino

The phenomenon of posttranscriptional gene silencing (PTGS), which occurs when a transgene is introduced into a cell, is poorly understood. Here, the qde-3 gene, which is required for the activation and maintenance of gene silencing in the fungus Neurospora crassa, was isolated. Sequence analysis revealed that the qde-3 gene belongs to the RecQ DNA helicase family. The QDE3 protein may function in the DNA-DNA interaction between introduced transgenes or with an endogenous gene required for gene-silencing activation. In animals, genes that are homologous to RecQ protein, such as the human genes for Bloom's syndrome and Werner's syndrome, may also function in PTGS.

Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Molecolare, Università di Roma La Sapienza, Viale Regina Elena, 324, 00161 Roma, Italy.
*   To whom correspondence should be addressed. E-mail: carlo{at}bce.med.uniroma1.it

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