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Science 17 December 1999:
Vol. 286. no. 5448, pp. 2339 - 2342
DOI: 10.1126/science.286.5448.2339
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Reports

Requirement of Yeast SGS1 and SRS2 Genes for Replication and Transcription

Sung-Keun Lee, Robert E. Johnson, Sung-Lim Yu, Louise Prakash, Satya Prakash *

The SGS1 gene of the yeast Saccharomyces cerevisiae encodes a DNA helicase with homology to the human Bloom's syndrome gene BLM and the Werner's syndrome gene WRN. The SRS2 gene of yeast also encodes a DNA helicase. Simultaneous deletion of SGS1 and SRS2 is lethal in yeast. Here, using a conditional mutation of SGS1, it is shown that DNA replication and RNA polymerase I transcription are drastically inhibited in the srs2Delta sgs1-ts strain at the restrictive temperature. Thus, SGS1 and SRS2 function in DNA replication and RNA polymerase I transcription. These functions may contribute to the various defects observed in Werner's and Bloom's syndromes.

Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, 6.104 Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061, USA.
*   To whom correspondence should be addressed. E-mail: sprakash{at}scms.utmb.edu

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